KIAA1161 CRISPR/Cas9 KO Plasmid (h): sc-412801
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KIAA1161 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KIAA1161 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KIAA1161 CRISPR/Cas9 KO Plasmid (h) | sc-412801 | 20 µg | $397.00 |
Overview
KIAA1161 encodes a large, poorly characterized human protein that is predicted to function in intracellular regulation through protein–protein interactions and subcellular compartmentalization. Expression and annotation patterns suggest involvement in fundamental cellular processes such as cytoskeletal organization, membrane trafficking, and signal integration, with potential downstream effects on cell cycle progression and stress-responsive transcriptional programs. Because many KIAA family proteins act as network hubs in RNA/protein complexes, perturbation of KIAA1161 is useful for mapping pathway connectivity and compensatory signaling. Altered expression of uncharacterized KIAA proteins has been reported across multiple disease contexts, making KIAA1161 a relevant target for mechanistic studies in cancer biology and other complex phenotypes without implying clinical utility.
KIAA1161 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KIAA1161 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KIAA1161 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KIAA1161 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KIAA1161 protein expression.
This CRISPR knockout system enables efficient generation of KIAA1161-deficient cell models for investigation of KIAA1161 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KIAA1161 exon(s) critical for KIAA1161 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KIAA1161 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KIAA1161 CRISPR/Cas9 KO Plasmid (h) and KIAA1161 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KIAA1161 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KIAA1161 HDR Plasmid (h) and KIAA1161 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KIAA1161 homology arms to support homology-directed repair at defined KIAA1161 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.