KIAA0319L CRISPR/Cas9 KO Plasmid (h): sc-413301
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KIAA0319L CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KIAA0319L genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KIAA0319L CRISPR/Cas9 KO Plasmid (h) | sc-413301 | 20 µg | $397.00 |
Overview
KIAA0319L encodes a transmembrane protein enriched at the cell surface and endosomal compartments, frequently studied for roles in cell adhesion, vesicular trafficking, and receptor-mediated endocytosis. It is considered functionally related to KIAA0319 family members implicated in neurodevelopmental processes, including neuronal migration and cortical organization. Cellular studies associate KIAA0319L with membrane dynamics and intracellular sorting pathways that influence signaling and cell–cell interactions. Genetic and functional evidence has linked variation in this locus to neurodevelopmental phenotypes, supporting its use as a target in mechanistic models of brain development and disease-relevant cellular behaviors.
KIAA0319L CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KIAA0319L gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KIAA0319L together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KIAA0319L open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KIAA0319L protein expression.
This CRISPR knockout system enables efficient generation of KIAA0319L-deficient cell models for investigation of KIAA0319L signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KIAA0319L exon(s) critical for KIAA0319L function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KIAA0319L genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KIAA0319L CRISPR/Cas9 KO Plasmid (h) and KIAA0319L CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KIAA0319L locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KIAA0319L HDR Plasmid (h) and KIAA0319L HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KIAA0319L homology arms to support homology-directed repair at defined KIAA0319L target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.