Keratin 81 Double Nickase Plasmid (h): sc-405797-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Keratin 81 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Keratin 81 Double Nickase Plasmid (h) and Keratin 81 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KRT81. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Keratin 81 Antibody (36-Z): sc-100929
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Keratin 81 Double Nickase Plasmid (h) | sc-405797-NIC | 20 µg | $410.00 | |||
Keratin 81 Double Nickase Plasmid (h2) | sc-405797-NIC-2 | 20 µg | $410.00 |
KRT81 encodes keratin 81, a type II hair keratin that assembles with type I keratins to form intermediate filaments providing tensile strength and structural organization in differentiated hair follicle epithelium. Keratin 81 contributes to cytoskeletal integrity, cellular mechanics, and the architecture of keratinized structures through regulated filament assembly, crosslinking, and differentiation-dependent expression. Its function is integrated with epithelial differentiation programs and stress-response signaling that influence filament dynamics and tissue resilience. Altered KRT81 sequence or expression is associated with hair shaft fragility phenotypes and related disorders of hair fiber formation, making it a relevant target for studying keratin network biology.
Keratin 81 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KRT81 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KRT81. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KRT81 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KRT81-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.