Keratin 74 Antibody (E-6): sc-390340

Keratin 74 Antibody (E-6) is a mouse monoclonal IgG3 kappa light chain antibody that detects Keratin 74 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Keratin 74 antibody (E-6) is available as the non-conjugated form. Keratin 74, also known as K6IRS4, KRT5C, or KRT6IRS4, is a 529 amino acid protein that plays a crucial role in the structural integrity of hair follicles, particularly in the Huxley layer of the inner root sheath. Keratin 74 is part of the keratin multigene family, which includes both "soft" epithelial cytokeratins and "hard" hair keratins, contributing to the formation and maintenance of epithelial tissues and hair structures. Proper functioning of Keratin 74 is essential for hair growth and health, as defects in Keratin 74 can lead to woolly hair autosomal dominant (ADWH), a rare condition characterized by fine, tightly curled hair that ceases to grow after a few inches, ultimately resulting in progressive hair loss beginning in early adulthood. The gene encoding Keratin 74 is located on human chromosome 12, highlighting its significance in genetic studies related to hair disorders. With exceptional detection capabilities in various applications, Keratin 74 (E-6) antibody serves as an invaluable tool for researchers investigating the molecular mechanisms underlying hair follicle biology and associated pathologies.

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Keratin 74 Antibody (E-6) References:

1. Characterization of a 300 kbp region of human DNA containing the type II hair keratin gene domain.  |  Rogers, MA., et al. 2000. J Invest Dermatol. 114: 464-72. PMID: 10692104
2. The catalog of human hair keratins. II. Expression of the six type II members in the hair follicle and the combined catalog of human type I and II keratins.  |  Langbein, L., et al. 2001. J Biol Chem. 276: 35123-32. PMID: 11445569
3. Genes for intermediate filament proteins and the draft sequence of the human genome: novel keratin genes and a surprisingly high number of pseudogenes related to keratin genes 8 and 18.  |  Hesse, M., et al. 2001. J Cell Sci. 114: 2569-75. PMID: 11683385
4. Characterization of new members of the human type II keratin gene family and a general evaluation of the keratin gene domain on chromosome 12q13.13.  |  Rogers, MA., et al. 2005. J Invest Dermatol. 124: 536-44. PMID: 15737194
5. Keratins of the human hair follicle.  |  Langbein, L. and Schweizer, J. 2005. Int Rev Cytol. 243: 1-78. PMID: 15797458
6. K25 (K25irs1), K26 (K25irs2), K27 (K25irs3), and K28 (K25irs4) represent the type I inner root sheath keratins of the human hair follicle.  |  Langbein, L., et al. 2006. J Invest Dermatol. 126: 2377-86. PMID: 16874310
7. Autosomal-dominant woolly hair resulting from disruption of keratin 74 (KRT74), a potential determinant of human hair texture.  |  Shimomura, Y., et al. 2010. Am J Hum Genet. 86: 632-8. PMID: 20346438
8. Novel mutations in the keratin-74 (KRT74) gene underlie autosomal dominant woolly hair/hypotrichosis in Pakistani families.  |  Wasif, N., et al. 2011. Hum Genet. 129: 419-24. PMID: 21188418
9. Sequences and differential expression of three novel human type-II hair keratins.  |  Rogers, MA., et al. 1997. Differentiation. 61: 187-94. PMID: 9084137

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Keratin 74 Antibody (E-6) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Keratin 74 Antibody (E-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Keratin 74 Antibody (E-6) Specifications

Alternate Product Name: Keratin 74

Product Clone Name: E-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 475-486

Epitope Target: Keratin 74

Epitope Species: human

Product Reactivity/Detection: Keratin 74

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Keratin 74 Antibody (E-6): P: Blocking Peptide