Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
KCTD15 Double Nickase Plasmid (h): sc-407663-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KCTD15 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- KCTD15 Double Nickase Plasmid (h) and KCTD15 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KCTD15. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: KCTD15 Antibody (AT4C3): sc-517410
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KCTD15 Double Nickase Plasmid (h) | sc-407663-NIC | 20 µg | $410.00 | |||
KCTD15 Double Nickase Plasmid (h2) | sc-407663-NIC-2 | 20 µg | $410.00 |
KCTD15 encodes a BTB/POZ domain–containing adaptor protein in the potassium channel tetramerization domain (KCTD) family that contributes to protein–protein interactions and regulation of signaling networks. KCTD15 has been linked to modulation of transcriptional programs governing neural crest development and adipogenesis, and is frequently discussed in the context of WNT/β-catenin–related regulatory circuitry and cellular differentiation states. Genetic association studies implicate KCTD15 loci in obesity-related traits, and altered KCTD15 expression has been reported across contexts relevant to metabolic regulation and neurodevelopment. These properties make KCTD15 a useful target for dissecting pathway cross-talk, lineage specification, and genotype-to-phenotype relationships in human cell models.
KCTD15 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KCTD15 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KCTD15. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KCTD15 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KCTD15-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.