KCC1 Antibody (FT-94): sc-134370

KCC1 Antibody (FT-94) is a mouse monoclonal IgG1 kappa light chain antibody that detects KCC1 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-KCC1 antibody (FT-94) is available as a non-conjugated format. KCC1, a member of the potassium/chloride co-transporters family, plays a crucial role in maintaining cell volume and regulating intracellular ion concentrations, which is vital for cellular homeostasis and function. KCC1 is characterized by 12 transmembrane domains, with both N and C terminal domains located in the cytoplasm, allowing effective transport of potassium and chloride ions across the cell membrane. This transport activity is particularly important in various physiological processes, including neuronal signaling and muscle contraction. KCC1 is ubiquitously expressed, which underscores its fundamental role in diverse tissues. The activation of KCCs by nitric oxide donors and inhibition of this activation by cGMP pathway blockers highlight the intricate regulatory mechanisms governing KCC1 function. Understanding KCC1 dynamics and interactions with other proteins can provide insights into contributions to pathophysiological conditions, including neurological disorders and muscle diseases.

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KCC1 Antibody (FT-94) References:

1. Cloning and characterization of KCC3 and KCC4, new members of the cation-chloride cotransporter gene family.  |  Mount, DB., et al. 1999. J Biol Chem. 274: 16355-62. PMID: 10347194
2. K-Cl cotransport: properties and molecular mechanism.  |  Lauf, PK. and Adragna, NC. 2000. Cell Physiol Biochem. 10: 341-54. PMID: 11125215
3. Neuronal restrictive silencing element is found in the KCC2 gene: molecular basis for KCC2-specific expression in neurons.  |  Karadsheh, MF. and Delpire, E. 2001. J Neurophysiol. 85: 995-7. PMID: 11160529
4. Localization of the K(+)-Cl(-) cotransporter, KCC3, in the central and peripheral nervous systems: expression in the choroid plexus, large neurons and white matter tracts.  |  Pearson, MM., et al. 2001. Neuroscience. 103: 481-91. PMID: 11246162
5. Protein kinase G regulates potassium chloride cotransporter-4 [corrected] expression in primary cultures of rat vascular smooth muscle cells.  |  Di Fulvio, M., et al. 2001. J Biol Chem. 276: 21046-52. PMID: 11274213
6. Mutation analysis of the potassium chloride cotransporter KCC3 (SLC12A6) in rolandic and idiopathic generalized epilepsy.  |  Steinlein, OK., et al. 2001. Epilepsy Res. 44: 191-5. PMID: 11325574
7. Disruption of KCC2 reveals an essential role of K-Cl cotransport already in early synaptic inhibition.  |  Hübner, CA., et al. 2001. Neuron. 30: 515-24. PMID: 11395011
8. K-Cl cotransport: immunohistochemical and ion flux studies in human embryonic kidney (HEK293) cells transfected with full-length and C-terminal-domain-truncated KCC1 cDNAs.  |  Lauf, PK., et al. 2001. Cell Physiol Biochem. 11: 143-60. PMID: 11410710
9. The mRNA level of the potassium-chloride cotransporter KCC2 covaries with seizure susceptibility in inferior colliculus of the post-ischemic audiogenic seizure-prone rat.  |  Reid, KH., et al. 2001. Neurosci Lett. 308: 29-32. PMID: 11445278
10. The differential expression patterns of messenger RNAs encoding K-Cl cotransporters (KCC1,2) and Na-K-2Cl cotransporter (NKCC1) in the rat nervous system.  |  Kanaka, C., et al. 2001. Neuroscience. 104: 933-46. PMID: 11457581