KBTBD6 CRISPR/Cas9 KO Plasmid (h): sc-409349
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KBTBD6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KBTBD6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KBTBD6 CRISPR/Cas9 KO Plasmid (h) | sc-409349 | 20 µg | $397.00 |
Overview
KBTBD6 (kelch repeat and BTB domain containing 6) encodes a BTB–BACK–Kelch family protein predicted to act as a substrate adaptor within CUL3-based E3 ubiquitin ligase complexes. Through BTB-mediated cullin binding and Kelch-dependent substrate recognition, KBTBD6 is positioned to regulate ubiquitination and proteasomal turnover of proteins controlling cell-cycle progression, cytoskeletal organization, and stress-responsive signaling. These protein quality-control processes influence pathways such as ubiquitin-dependent degradation and associated transcriptional and proliferative programs. Dysregulation of ubiquitin ligase adaptors is frequently linked to altered proteostasis and signaling states observed in cancer and other disorders, making KBTBD6 relevant for mechanistic studies of ubiquitin-mediated regulation.
KBTBD6 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KBTBD6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KBTBD6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KBTBD6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KBTBD6 protein expression.
This CRISPR knockout system enables efficient generation of KBTBD6-deficient cell models for investigation of KBTBD6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KBTBD6 exon(s) critical for KBTBD6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KBTBD6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KBTBD6 CRISPR/Cas9 KO Plasmid (h) and KBTBD6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KBTBD6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KBTBD6 HDR Plasmid (h) and KBTBD6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KBTBD6 homology arms to support homology-directed repair at defined KBTBD6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.