Katanin p60 AL1 Antibody (A-10): sc-373814

Katanin p60 AL1 Antibody (A-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects Katanin p60 AL1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Katanin p60 AL1 antibody (A-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Katanin p60 AL1 plays a crucial role in microtubule dynamics, which are essential for various cellular processes, including cell division and intracellular transport. Specifically, Katanin p60 AL1 is part of a heterodimeric complex that severs microtubules in an ATP-dependent manner, facilitating reorganization of microtubule arrays and release of microtubules from the centrosome after nucleation. This severing activity is vital for maintaining proper cellular architecture and function, as dynamic remodeling of the cytoskeleton responds to cellular needs. The Katanin complex consists of the 60 kDa subunit (Katanin p60 A1) and an 80 kDa accessory protein (Katanin p80 B1), where Katanin p60 A1 performs the severing action, while Katanin p80 B1 directs the complex to the centrosome. Both Katanin p60 A1 and Katanin p80 B1 belong to the AAA ATPase family, which also includes Katanin p60 A1-like proteins, Katanin p60 AL1 and Katanin p60 AL2, highlighting evolutionary conservation and functional importance in cellular processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Katanin p60 AL1 Antibody (A-10) References:

1. An essential role for katanin in severing microtubules in the neuron.  |  Ahmad, FJ., et al. 1999. J Cell Biol. 145: 305-15. PMID: 10209026
2. Two domains of p80 katanin regulate microtubule severing and spindle pole targeting by p60 katanin.  |  McNally, KP., et al. 2000. J Cell Sci. 113 (Pt 9): 1623-33. PMID: 10751153
3. Axonal growth is sensitive to the levels of katanin, a protein that severs microtubules.  |  Karabay, A., et al. 2004. J Neurosci. 24: 5778-88. PMID: 15215300
4. Recruitment of katanin p60 by phosphorylated NDEL1, an LIS1 interacting protein, is essential for mitotic cell division and neuronal migration.  |  Toyo-Oka, K., et al. 2005. Hum Mol Genet. 14: 3113-28. PMID: 16203747
5. LAPSER1/LZTS2: a pluripotent tumor suppressor linked to the inhibition of katanin-mediated microtubule severing.  |  Sudo, H. and Maru, Y. 2008. Hum Mol Genet. 17: 2524-40. PMID: 18490357
6. Identification of katanin, an ATPase that severs and disassembles stable microtubules.  |  McNally, FJ. and Vale, RD. 1993. Cell. 75: 419-29. PMID: 8221885
7. Katanin, the microtubule-severing ATPase, is concentrated at centrosomes.  |  McNally, FJ., et al. 1996. J Cell Sci. 109 (Pt 3): 561-7. PMID: 8907702
8. Katanin, a microtubule-severing protein, is a novel AAA ATPase that targets to the centrosome using a WD40-containing subunit.  |  Hartman, JJ., et al. 1998. Cell. 93: 277-87. PMID: 9568719

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Katanin p60 AL1 Antibody (A-10) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-Katanin p60 AL1 Antibody (A-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Katanin p60 AL1 Antibody (A-10) Specifications

Alternate Product Name: Katanin p60 AL1

Product Clone Name: A-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 72-109

Epitope Target: Katanin p60 AL1

Epitope Species: human

Product Reactivity/Detection: Katanin p60 AL1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for Katanin p60 AL1 Antibody (A-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin