karyopherin alpha 6 Antibody (E-11) recommended for detection of karyopherin α6 of mouse, rat and human origin by WB, IP, IF and ELISA

karyopherin α6 Antibody (E-11): sc-390055

karyopherin α6 Antibody (E-11) is rated 4.7 out of 5 by 3.
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Datasheets

    • karyopherin α6 Antibody (E-11) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 51-104 mapping near the N-terminus of karyopherin α6 of human origin
    • Anti-karyopherin alpha 6 Antibody (E-11) is recommended for detection of karyopherin α6 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine and porcine
    • Anti-karyopherin alpha 6 Antibody (E-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of karyopherin α6 (E-11): sc-390055.

What is the recommended starting dilution for Western blot for sc-390055: karyopherin α6 (E-11) monoclonal antibody?

Asked by: Germaine
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from good product Very strong and clean signal when used in 1:2000 dilution in Hela cells.
Date published: 2018-08-22
Rated 4 out of 5 by from A good antibody to detect KPNA6 We bought this antibody to detect KPNA6 in cultured HeLa and Vero cells. It works well in Western blotting and immunofluorescence assay.
Date published: 2018-07-18
Rated 5 out of 5 by from Produced positive Western blot data of karyopherin Produced positive Western blot data of karyopherin 6 expression in HeLa, K-562, Hep G2, Caki-1, ES-2 and HuT 78 whole cell lysates. -SCBT QC
Date published: 2014-08-12
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