karyopherin α6 Antibody (E-11) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
raised against amino acids 51-104 mapping near the N-terminus of karyopherin α6 of human origin
Anti-karyopherin alpha 6 Antibody (E-11) is recommended for detection of karyopherin α6 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine and porcine
Anti-karyopherin alpha 6 Antibody (E-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of karyopherin α6 (E-11): sc-390055.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Protein transport across the nucleus is a selective, multi-step process involving several cytoplasmic factors that mediate protein passage through the nuclear pore complex (NPC). Cytoplasmic proteins that contain nuclear localization signals (NLSs) must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Karyopherin α1 and karyopherin α6 are widely expressed nuclear import proteins that act as adaptors for karyopherin β1, specifically binding to and guiding NLS-containing proteins to the NPC. Both karyopherin α1 and karyopherin α6 contain one IBB domain and ten ARM repeats through which they convey their protein binding and localization function. Together, karyopherin α1 and karyopherin α6 are responsible for ensuring the nuclear import of NLS-containing substrates.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What is the recommended starting dilution for Western blot for sc-390055: karyopherin α6 (E-11) monoclonal antibody?
Asked by: Germaine
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol:
Rated 5 out of
good productVery strong and clean signal when used in 1:2000 dilution in Hela cells.
Date published: 2018-08-22
Rated 4 out of
A good antibody to detect KPNA6We bought this antibody to detect KPNA6 in cultured HeLa and Vero cells. It works well in Western blotting and immunofluorescence assay.
Date published: 2018-07-18
Rated 5 out of
Produced positive Western blot data of karyopherinProduced positive Western blot data of karyopherin 6 expression in HeLa, K-562, Hep G2, Caki-1, ES-2 and HuT 78 whole cell lysates. -SCBT QC
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