karyopherin α3 Antibody (B-1): sc-514101

karyopherin α3 Antibody (B-1) is a mouse monoclonal IgG1 antibody that detects karyopherin alpha 3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-karyopherin alpha 3 antibody (B-1) is available as the non-conjugated form. Karyopherin α3, also known as KPNA3 or QIP2, is a crucial protein involved in the selective transport of proteins across the nuclear envelope, a process essential for maintaining cellular function and regulating gene expression. This 521 amino acid protein is characterized by localization in both the nucleus and cytoplasm, containing one importin beta-binding (IBB) domain and ten armadillo (ARM) repeats, which facilitate interaction with nuclear localization signal (NLS)-containing proteins. Karyopherin α3 plays a pivotal role in recognizing these proteins, docking them at the nuclear pore complex, and mediating their ATP-dependent translocation into the nucleus. Notably, karyopherin α3 is expressed ubiquitously, with the highest levels found in heart and skeletal muscle tissues, and also interacts with HIV-1 proteins, highlighting importance in viral pathogenesis. Furthermore, karyopherin α3 serves as an adaptor for karyopherin β1, enhancing the docking of target substrates to the nuclear pore complex, thereby influencing the overall efficiency of nuclear transport. The gene encoding karyopherin α3 is located on human chromosome 13 and has been implicated in the pathogenesis of schizophrenia, making karyopherin α3 a significant target for research in neurobiology and virology.

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karyopherin α3 Antibody (B-1) References:

1. Evidence for distinct substrate specificities of importin alpha family members in nuclear protein import.  |  Köhler, M., et al. 1999. Mol Cell Biol. 19: 7782-91. PMID: 10523667
2. The nuclear import of RCC1 requires a specific nuclear localization sequence receptor, karyopherin alpha3/Qip.  |  Talcott, B. and Moore, MS. 2000. J Biol Chem. 275: 10099-104. PMID: 10744690
3. Developmental regulation of the heat shock response by nuclear transport factor karyopherin-alpha3.  |  Fang, X., et al. 2001. Development. 128: 3349-58. PMID: 11546751
4. A synthetic peptide bearing the HIV-1 integrase 161-173 amino acid residues mediates active nuclear import and binding to importin alpha: characterization of a functional nuclear localization signal.  |  Armon-Omer, A., et al. 2004. J Mol Biol. 336: 1117-28. PMID: 15037073
5. Nucleolar proteome dynamics.  |  Andersen, JS., et al. 2005. Nature. 433: 77-83. PMID: 15635413
6. The KPNA3 gene may be a susceptibility candidate for schizophrenia.  |  Wei, J. and Hemmings, GP. 2005. Neurosci Res. 52: 342-6. PMID: 15882913
7. The two steps of nuclear import, targeting to the nuclear envelope and translocation through the nuclear pore, require different cytosolic factors.  |  Moore, MS. and Blobel, G. 1992. Cell. 69: 939-50. PMID: 1606616
8. Dominant KPNA3 Mutations Cause Infantile-Onset Hereditary Spastic Paraplegia.  |  Schob, C., et al. 2021. Ann Neurol. 90: 738-750. PMID: 34564892
9. KPNA3 promotes epithelial-mesenchymal transition by regulating TGF-β and AKT signaling pathways in MDA-MB-231, a triple-negative breast cancer cell line.  |  Choi, J., et al. 2023. BMB Rep. 56: 120-125. PMID: 36593106
10. Role of the karyopherin pathway in human immunodeficiency virus type 1 nuclear import.  |  Gallay, P., et al. 1996. J Virol. 70: 1027-32. PMID: 8551560
11. Isolation and mapping of karyopherin alpha 3 (KPNA3), a human gene that is highly homologous to genes encoding Xenopus importin, yeast SRP1 and human RCH1.  |  Takeda, S., et al. 1997. Cytogenet Cell Genet. 76: 87-93. PMID: 9154134
12. Cloning of two novel human importin-alpha subunits and analysis of the expression pattern of the importin-alpha protein family.  |  Köhler, M., et al. 1997. FEBS Lett. 417: 104-8. PMID: 9395085

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-karyopherin α3 Antibody (B-1) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-karyopherin α3 Antibody (B-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

karyopherin α3 Antibody (B-1) Specifications

Alternate Product Name: karyopherin α3

Product Clone Name: B-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 51-90

Epitope Target: karyopherin α3

Epitope Species: human

Product Reactivity/Detection: karyopherin α3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for karyopherin α3 Antibody (B-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin