karyopherin α2 Antibody (B-9): sc-55538

karyopherin α2 Antibody (B-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects karyopherin alpha 2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-karyopherin α2 antibody (B-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. karyopherin alpha 2 plays a crucial role in nuclear transport of proteins, specifically in recognizing and binding to nuclear localization signals on substrates destined for import into the nucleus. This process maintains cellular function by regulating transport of proteins essential for gene expression, cell cycle progression, and signal transduction. karyopherin alpha 2 is primarily located in the cytoplasm and nucleus, where karyopherin alpha 2 interacts with other transport factors and nucleoporins to facilitate protein translocation across the nuclear envelope. karyopherin alpha 2 has also been shown to interact with Epstein-Barr virus nuclear antigen 1 (EBNA1), highlighting importance not only in cellular processes but also in viral pathogenesis. The ability of karyopherin alpha 2 to mediate protein import underscores significance in both normal cellular function and disease states, making anti-karyopherin α2 antibody (B-9) an essential tool for researchers studying nuclear transport mechanisms and their implications in health and disease.

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karyopherin α2 Antibody (B-9) References:

1. Karyopherin-alpha2 protein interacts with Chk2 and contributes to its nuclear import.  |  Zannini, L., et al. 2003. J Biol Chem. 278: 42346-51. PMID: 12909615
2. Karyopherin α2 induces apoptosis in tongue squamous cell carcinoma CAL-27 cells through the p53 pathway.  |  Gao, L., et al. 2016. Oncol Rep. 35: 3357-62. PMID: 27109484
3. Enhanced karyopherin-α2 expression is associated with carcinogenesis in patients with intraductal papillary mucinous neoplasms.  |  Kubo, N., et al. 2017. Pancreatology. 17: 611-616. PMID: 28476582
4. Long non-coding RNA HAGLROS facilitates tumorigenesis and progression in hepatocellular carcinoma by sponging miR-26b-5p to up-regulate karyopherin α2 (KPNA2) and inactivate p53 signaling.  |  Tang, G., et al. 2022. Bioengineered. 13: 7829-7846. PMID: 35291921
5. Protein export from the nucleus requires the GTPase Ran and GTP hydrolysis.  |  Moroianu, J. and Blobel, G. 1995. Proc Natl Acad Sci U S A. 92: 4318-22. PMID: 7753805
6. Previously identified protein of uncertain function is karyopherin alpha and together with karyopherin beta docks import substrate at nuclear pore complexes.  |  Moroianu, J., et al. 1995. Proc Natl Acad Sci U S A. 92: 2008-11. PMID: 7892216
7. Ran binding domains promote the interaction of Ran with p97/beta-karyopherin, linking the docking and translocation steps of nuclear import.  |  Lounsbury, KM., et al. 1996. J Biol Chem. 271: 2357-60. PMID: 8576188
8. The binding site of karyopherin alpha for karyopherin beta overlaps with a nuclear localization sequence.  |  Moroianu, J., et al. 1996. Proc Natl Acad Sci U S A. 93: 6572-6. PMID: 8692858
9. Nuclear protein import: Ran-GTP dissociates the karyopherin alphabeta heterodimer by displacing alpha from an overlapping binding site on beta.  |  Moroianu, J., et al. 1996. Proc Natl Acad Sci U S A. 93: 7059-62. PMID: 8692944
10. Epstein-Barr virus nuclear antigen 1 forms a complex with the nuclear transporter karyopherin alpha2.  |  Fischer, N., et al. 1997. J Biol Chem. 272: 3999-4005. PMID: 9020106
11. Cloning and characterization of human karyopherin beta3.  |  Yaseen, NR. and Blobel, G. 1997. Proc Natl Acad Sci U S A. 94: 4451-6. PMID: 9114010
12. Karyopherin beta2 mediates nuclear import of a mRNA binding protein.  |  Bonifaci, N., et al. 1997. Proc Natl Acad Sci U S A. 94: 5055-60. PMID: 9144189

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-karyopherin α2 Antibody (B-9) has 26 citations in a variety of scientific publications.

Primary antibodies like Anti-karyopherin α2 Antibody (B-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

karyopherin α2 Antibody (B-9) Specifications

Alternate Product Name: karyopherin α2

Product Clone Name: B-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 480-529

Epitope Target: karyopherin α2

Epitope Species: human

Product Reactivity/Detection: karyopherin α2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 26 citations in various scientific publications

Available Conjugates/Forms for karyopherin α2 Antibody (B-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin