karyopherin alpha 2 Antibody (2G7) recommended for detection of karyopherin α2 of mouse, rat and human origin by WB and IP

karyopherin α2 Antibody (2G7): sc-57009

karyopherin α2 Antibody (2G7) is rated 4.8 out of 5 by 4.
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Datasheets

    • karyopherin α2 Antibody (2G7) is a rat monoclonal IgG2a provided at 200 µg/ml
    • raised against amino acids 116-515 of karyopherin α2 of human origin
    • recommended for detection of karyopherin α2 of mouse, rat and human origin by WB and IP
    • See karyopherin α2 (B-9): sc-55538 for karyopherin α2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

How much sample do you recommend loading on the gel for Western blot detection using karyopherin α2 (2G7): sc-57009?

Asked by: jerojero
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from WB: robust band in BJAB whole cell lysate WB: robust band in BJAB whole cell lysate. -SCBT QC
Date published: 2015-06-17
Rated 5 out of 5 by from Positive results in HeLa whole cell lysate Positive results in HeLa whole cell lysate with western blotting. -SCBT QC
Date published: 2015-02-22
Rated 4 out of 5 by from Good specificity and signal strength with Good specificity and signal strength with some background bands in western blot with Jurkat and transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-02-01
Rated 5 out of 5 by from Strong bands in western blot analysis of Strong bands in western blot analysis of karyopherin 2 expression in HeLa and MCF7 nuclear extracts. -SCBT QC
Date published: 2014-02-11
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