Kaptin Antibody (3B1): sc-100918

Kaptin Antibody (3B1) is a mouse monoclonal IgG2a kappa light chain antibody that detects the Kaptin protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Kaptin antibody (3B1) is available as the non-conjugated form. Kaptin, also known as 2E4, plays a crucial role in the dynamic processes of platelet activation, which is essential for hemostasis. Kaptin binds to filamentous (F)-actin in an ATP-dependent manner, facilitating the polymerization of actin into various structures such as filopodia and stress fibers during platelet activation. Kaptin (3B1) localizes at the leading edge of platelets, underscoring its importance in actin rearrangements that occur during platelet activation. Additionally, Kaptin is found at the leading edge of elongating stereocilia in the inner ear, where Kaptin functions integrally in the mechanotransduction of sound. Kaptin′s involvement in stereocilia formation highlights its potential role in the development of DFNA4, a form of autosomal dominant non-syndromic hearing loss. Kaptin′s ability to interact with actin and presence in critical cellular structures make Kaptin a significant protein for understanding both platelet function and auditory mechanisms.

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Kaptin Antibody (3B1) References:

1. 2E4 (kaptin): a novel actin-associated protein from human blood platelets found in lamellipodia and the tips of the stereocilia of the inner ear.  |  Bearer, EL. and Abraham, MT. 1999. Eur J Cell Biol. 78: 117-26. PMID: 10099934
2. Cortactin localization to sites of actin assembly in lamellipodia requires interactions with F-actin and the Arp2/3 complex.  |  Weed, SA., et al. 2000. J Cell Biol. 151: 29-40. PMID: 11018051
3. 2E4/Kaptin (KPTN)--a candidate gene for the hearing loss locus, DFNA4.  |  Bearer, EL., et al. 2000. Ann Hum Genet. 64: 189-96. PMID: 11409409
4. Actin dynamics in platelets.  |  Bearer, EL., et al. 2002. Int Rev Cytol. 217: 137-82. PMID: 12019562
5. Mechanisms of actin rearrangements mediating platelet activation.  |  Hartwig, JH. 1992. J Cell Biol. 118: 1421-42. PMID: 1325975
6. An actin-associated protein present in the microtubule organizing center and the growth cones of PC-12 cells.  |  Bearer, EL. 1992. J Neurosci. 12: 750-61. PMID: 1372044
7. Cytoskeletal domains in the activated platelet.  |  Bearer, EL. 1995. Cell Motil Cytoskeleton. 30: 50-66. PMID: 7728868