κB-Ras1 Antibody (E-3): sc-271169
- κB-Ras1 Antibody (E-3) is a mouse monoclonal IgG1 κ κB-Ras1 antibody provided at 200 µg/ml
- Anti-kappa B-Ras1 Antibody (E-3) is recommended for detection of κB-Ras1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- kappa B-Ras1 Antibody (E-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for κB-Ras1 Antibody (E-3) for WB and IHC(P) applications. These reagents are now offered in bundles with κB-Ras1 Antibody (E-3) (see ordering information below).
κB-Ras1 Antibody (E-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects κB-Ras1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). κB-Ras1 (E-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. κB-Ras1 protein plays a crucial role in regulating cellular signaling pathways, particularly in the NF-κB signaling cascade, which is vital for immune response, cell proliferation, and survival. Located primarily in the cytoplasm, κB-Ras1 interacts with IκB proteins, specifically IκBα and IκBβ, to modulate their degradation rates, thereby influencing NF-κB activation. Such interaction helps maintain cellular homeostasis and prevents excessive inflammatory responses, which can lead to various diseases, including cancer. Notably, κB-Ras1 and its closely related counterpart, κB-Ras2, share a high degree of sequence identity, with κB-Ras2 showing 71% similarity to κB-Ras1, underscoring their functional redundancy and importance in cellular signaling.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
κB-Ras1 Antibody (E-3) References:
- A subclass of Ras proteins that regulate the degradation of IkappaB. | Fenwick, C., et al. 2000. Science. 287: 869-73. PMID: 10657303
- Inhibition of NF-kappaB activity by IkappaBbeta in association with kappaB-Ras. | Chen, Y., et al. 2004. Mol Cell Biol. 24: 3048-56. PMID: 15024091
- ras oncogenes in human cancer: a review. | Bos, JL. 1989. Cancer Res. 49: 4682-9. PMID: 2547513
- The ras gene family and human carcinogenesis. | Bos, JL. 1988. Mutat Res. 195: 255-71. PMID: 3283542
- Activators and effectors of ras p21 proteins. | McCormick, F. 1994. Curr Opin Genet Dev. 4: 71-6. PMID: 8193543
- Signal transduction through NF-kappa B. | May, MJ. and Ghosh, S. 1998. Immunol Today. 19: 80-8. PMID: 9509763
- All in the family? New insights and questions regarding interconnectivity of Ras, Rap1 and Ral. | Bos, JL. 1998. EMBO J. 17: 6776-82. PMID: 9843482
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
κB-Ras1 Antibody (E-3) | sc-271169 | 200 µg/ml | $316.00 | |||
κB-Ras1 Antibody (E-3): m-IgG Fc BP-HRP Bundle | sc-527215 | 200 µg Ab; 10 µg BP | $354.00 | |||
κB-Ras1 Antibody (E-3): m-IgG1 BP-HRP Bundle | sc-532588 | 200 µg Ab; 20 µg BP | $354.00 | |||
κB-Ras1 Antibody (E-3) AC | sc-271169 AC | 500 µg/ml, 25% agarose | $416.00 | |||
κB-Ras1 Antibody (E-3) HRP | sc-271169 HRP | 200 µg/ml | $316.00 | |||
κB-Ras1 Antibody (E-3) FITC | sc-271169 FITC | 200 µg/ml | $330.00 | |||
κB-Ras1 Antibody (E-3) PE | sc-271169 PE | 200 µg/ml | $343.00 | |||
κB-Ras1 Antibody (E-3) Alexa Fluor® 488 | sc-271169 AF488 | 200 µg/ml | $357.00 | |||
κB-Ras1 Antibody (E-3) Alexa Fluor® 546 | sc-271169 AF546 | 200 µg/ml | $357.00 | |||
κB-Ras1 Antibody (E-3) Alexa Fluor® 594 | sc-271169 AF594 | 200 µg/ml | $357.00 | |||
κB-Ras1 Antibody (E-3) Alexa Fluor® 647 | sc-271169 AF647 | 200 µg/ml | $357.00 | |||
κB-Ras1 Antibody (E-3) Alexa Fluor® 680 | sc-271169 AF680 | 200 µg/ml | $357.00 | |||
κB-Ras1 Antibody (E-3) Alexa Fluor® 790 | sc-271169 AF790 | 200 µg/ml | $357.00 |