JunD Antibody (D-9): sc-271938

Jun D Antibody (D-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects JunD protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). JunD (D-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. JunD protein plays a crucial role as a member of the activator protein-1 (AP-1) transcription factor family, which is essential for regulating gene expression in response to various stimuli, including growth factors and stress signals. JunD protein is primarily located in the nucleus, where JunD forms either homodimers or heterodimers with other Jun and Fos family members, allowing binding to specific DNA sequences known as the TGACTCA consensus sequence. This binding is vital for transcriptional regulation of genes involved in cell proliferation, differentiation, and apoptosis, making JunD a key player in cellular responses to external signals. Notably, structural similarities between JunD and c-Jun, particularly in their C-terminal regions, facilitate dimerization and DNA binding, while divergent N-terminal domains contribute to unique transcriptional activation properties. Furthermore, two forms of JunD may arise from internal initiation of translation, highlighting the complexity of regulation and function in cellular processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

JunD Antibody (D-9) References:

1. JunD protects cells from p53-dependent senescence and apoptosis.  |  Weitzman, JB., et al. 2000. Mol Cell. 6: 1109-19. PMID: 11106750
2. Expression of c-JUN, JUN B and JUN D proteins in rat nervous system following transection of vagus nerve and cervical sympathetic trunk.  |  Herdegen, T., et al. 1991. Neuroscience. 45: 413-22. PMID: 1762686
3. Expression of c-jun, jun B and jun D proto-oncogenes in human peripheral-blood granulocytes.  |  Mollinedo, F., et al. 1991. Biochem J. 273(Pt 2): 477-9. PMID: 1899335
4. Overexpression of c-jun, junB, or junD affects cell growth differently.  |  Castellazzi, M., et al. 1991. Proc Natl Acad Sci U S A. 88: 8890-4. PMID: 1924349
5. jun: oncogene and transcription factor.  |  Vogt, PK. and Bos, TJ. 1990. Adv Cancer Res. 55: 1-35. PMID: 2166997
6. jun-D: a third member of the jun gene family.  |  Ryder, K., et al. 1989. Proc Natl Acad Sci U S A. 86: 1500-3. PMID: 2493644
7. The product of a fos-related gene, fra-1, binds cooperatively to the AP-1 site with Jun: transcription factor AP-1 is comprised of multiple protein complexes.  |  Cohen, DR., et al. 1989. Genes Dev. 3: 173-84. PMID: 2497053
8. Characterization of junD: a new member of the jun proto-oncogene family.  |  Hirai, SI., et al. 1989. EMBO J. 8: 1433-9. PMID: 2504580
9. Fos and Jun: the AP-1 connection.  |  Curran, T. and Franza, BR. 1988. Cell. 55: 395-7. PMID: 3141060
10. Induction of protooncogene c-jun by serum growth factors.  |  Ryder, K. and Nathans, D. 1988. Proc Natl Acad Sci U S A. 85: 8464-7. PMID: 3186736
11. The antiestrogen tamoxifen induces c-fos and jun-B, but not c-jun or jun-D, protooncogenes in the rat uterus.  |  Nephew, KP., et al. 1993. Endocrinology. 133: 419-22. PMID: 8319588
12. Variations in Jun and Fos protein expression and AP-1 activity in cycling, resting and stimulated fibroblasts.  |  Lallemand, D., et al. 1997. Oncogene. 14: 819-30. PMID: 9047389

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-JunD Antibody (D-9) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-JunD Antibody (D-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

JunD Antibody (D-9) Specifications

Alternate Product Name: Jun D

Product Clone Name: D-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 316-341

Epitope Target: Jun D

Epitope Species: mouse

Product Reactivity/Detection: Jun D

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for JunD Antibody (D-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent

The preferred p-c-Jun antibody is p-c-Jun Antibody (KM-1): sc-822.