JP-45 Antibody (C-4): sc-377298

JP-45 Antibody (C-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects JP-45 of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). JP-45 (C-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. JP-45, also known as junctional sarcoplasmic reticulum protein 1 (JSRP1), is a 331 amino acid membrane protein located in the sarcoplasmic and endoplasmic reticulum. Strategic positioning within these organelles is crucial for JP-45′s role in calcium signaling, particularly in muscle cells, where JP-45 interacts with key proteins such as L-type calcium channel alpha-1S, L-type calcium channel beta-1B, and calsequestrin. These interactions are vital for regulating calcium influx during muscle contraction, thereby influencing excitation-contraction coupling. Proper functioning of JP-45 is essential for skeletal muscle development and maintenance, as disruptions in JP-45 expression can lead to decreased muscle strength, highlighting JP-45′s importance in muscle physiology. The gene encoding JP-45 is located on human chromosome 19p13.3, and while silencing JP-45 does not affect postnatal development, JP-45 silencing may compromise muscle function.

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JP-45 Antibody (C-4) References:

1. Identification of a novel 45 kDa protein (JP-45) from rabbit sarcoplasmic-reticulum junctional-face membrane.  |  Zorzato, F., et al. 2000. Biochem J. 351 Pt 2: 537-43. PMID: 11023841
2. The novel skeletal muscle sarcoplasmic reticulum JP-45 protein. Molecular cloning, tissue distribution, developmental expression, and interaction with alpha 1.1 subunit of the voltage-gated calcium channel.  |  Anderson, AA., et al. 2003. J Biol Chem. 278: 39987-92. PMID: 12871958
3. A possible role of the junctional face protein JP-45 in modulating Ca2+ release in skeletal muscle.  |  Gouadon, E., et al. 2006. J Physiol. 572: 269-80. PMID: 16423849
4. Calcium signalling in muscle: a milestone for modulation studies.  |  Ríos, E. 2006. J Physiol. 572: 1-2. PMID: 16469777
5. The junctional SR protein JP-45 affects the functional expression of the voltage-dependent Ca2+ channel Cav1.1.  |  Anderson, AA., et al. 2006. J Cell Sci. 119: 2145-55. PMID: 16638807
6. Loss of skeletal muscle strength by ablation of the sarcoplasmic reticulum protein JP45.  |  Delbono, O., et al. 2007. Proc Natl Acad Sci U S A. 104: 20108-13. PMID: 18077436
7. Follow-up of potential novel Graves' disease susceptibility loci, identified in the UK WTCCC genome-wide nonsynonymous SNP study.  |  Newby, PR., et al. 2010. Eur J Hum Genet. 18: 1021-6. PMID: 20442750

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-JP-45 Antibody (C-4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-JP-45 Antibody (C-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

JP-45 Antibody (C-4) Specifications

Alternate Product Name: JP-45

Product Clone Name: C-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 191-223

Epitope Target: JP-45

Epitope Species: mouse

Product Reactivity/Detection: JP-45

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for JP-45 Antibody (C-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin