JNK3 Antibody (10E4A4): sc-130075

JNK3 Antibody (10E4A4) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 28-233 of human JNK3, a stress-activated protein kinase involved in cellular stress responses. JNK3 mouse monoclonal antibody (10E4A4) recognizes an epitope within this specified region and demonstrates broad species cross-reactivity, detecting JNK3 in mouse, rat, and human samples through Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) applications. Anti-JNK3 monoclonal antibody (10E4A4) is available in non-conjugated form and multiple conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, offering versatility for diverse experimental needs. JNK3 belongs to a sophisticated family of kinases that enhance c-Jun′s transcriptional activity through phosphorylation. The complexity of JNK signaling stems from its elaborate gene structure: three distinct genes generate ten isoforms through alternative mRNA splicing. These isoforms include five p46 variants (JNK1a1, JNK1b1, JNK2a1, JNK2b1, and JNK3a1) and five p54 variants (JNK1a2, JNK1b2, JNK2a2, JNK2b2, and JNK3b2). JNK3 (also known as MK10, MAPK10) responds specifically to pro-inflammatory cytokines and environmental stressors, playing a critical role in neuronal apoptosis and stress-induced cell death. This specialized function makes JNK3 particularly significant in neurodegenerative conditions like Alzheimer′s and Parkinson′s diseases. Beyond neurological roles, JNK3 contributes to various cancers, including melanoma, basal cell carcinoma, and squamous cell carcinoma, highlighting JNK3′s importance in both tumorigenesis and immune responses. Through JNK3′s diverse splice variants and substrate interactions, JNK3 modulates multiple cellular processes, making JNK3 (10E4A4) antibody valuable for investigating both normal signaling mechanisms and disease pathways.

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JNK3 Antibody (10E4A4) References:

1. Inhibition of c-Jun N-terminal kinase 2 expression suppresses growth and induces apoptosis of human tumor cells in a p53-dependent manner.  |  Potapova, O., et al. 2000. Mol Cell Biol. 20: 1713-22. PMID: 10669748
2. Activation of JNK3 alpha 1 requires both MKK4 and MKK7: kinetic characterization of in vitro phosphorylated JNK3 alpha 1.  |  Lisnock, J., et al. 2000. Biochemistry. 39: 3141-8. PMID: 10715136
3. JNK is required for effector T-cell function but not for T-cell activation.  |  Dong, C., et al. 2000. Nature. 405: 91-4. PMID: 10811224
4. Isoforms of Jun kinase are differentially expressed and activated in human monocyte/macrophage (THP-1) cells.  |  Dreskin, SC., et al. 2001. J Immunol. 166: 5646-53. PMID: 11313405
5. Induced proliferation of human MRC-5 cells by nitrogen oxides via direct and indirect activation of MEKK1, JNK, and p38 signals.  |  Chou, FP., et al. 2002. Toxicol Appl Pharmacol. 181: 203-8. PMID: 12079429
6. Differential gene regulation by specific gain-of-function JNK1 proteins expressed in Swiss 3T3 fibroblasts.  |  Han, SY., et al. 2002. J Biol Chem. 277: 47167-74. PMID: 12354774
7. Selective interaction of JNK protein kinase isoforms with transcription factors.  |  Gupta, S., et al. 1996. EMBO J. 15: 2760-70. PMID: 8654373
8. Crystal structure of JNK3: a kinase implicated in neuronal apoptosis.  |  Xie, X., et al. 1998. Structure. 6: 983-91. PMID: 9739089
9. Defective T cell differentiation in the absence of Jnk1.  |  Dong, C., et al. 1998. Science. 282: 2092-5. PMID: 9851932

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-JNK3 Antibody (10E4A4) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-JNK3 Antibody (10E4A4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

JNK3 Antibody (10E4A4) Specifications

Alternate Product Name: JNK3

Product Clone Name: 10E4A4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 28-233

Epitope Target: JNK3

Epitope Species: human

Product Reactivity/Detection: JNK3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for JNK3 Antibody (10E4A4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred p-JNK antibody is p-JNK Antibody (G-7): sc-6254.