JNK1/3 Antibody (F-5): sc-514539

JNK1/3 Antibody (F-5) is a mouse monoclonal IgG3 kappa light chain antibody raised against amino acids 364-385 at the C-terminus of human JNK1 p46, a stress-activated protein kinase crucial for cellular homeostasis. JNK1/3 mouse monoclonal antibody (F-5) recognizes an epitope within this C-terminal region and demonstrates broad species cross-reactivity, detecting JNK1/3 in mouse, rat, and human samples through Western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA) applications. The sophistication of JNK signaling emerges through its complex molecular architecture: three genes generate ten distinct isoforms through alternative mRNA splicing. These include five p46 variants (JNK1a1, JNK1b1, JNK2a1, JNK2b1, JNK3a1) and five p54 variants (JNK1a2, JNK1b2, JNK2a2, JNK2b2, JNK3b2), each contributing to the intricate regulation of cellular stress responses. JNK1/3′s subcellular distribution reflects their functional versatility, while predominantly cytoplasmic, these kinases can translocate to the nucleus upon activation, enabling direct regulation of transcriptional responses. This spatial control becomes particularly important in cancer biology, where dysregulated JNK signaling contributes to various malignancies including breast, liver, and prostate cancers. JNK1 specifically demonstrates context-dependent functions in cancer, either promoting cell proliferation or inducing apoptosis depending on the cellular environment. Beyond cancer regulation, JNK1 plays a crucial role in immune system function, facilitating the activation and differentiation of CD4+ helper T cells into TH1 and TH2 effector cells. JNK1/3 respond to diverse cellular stressors: capsaicin triggers JNK1 activation alongside p38 in ras-transformed breast epithelial cells, while nitrogen oxides (NOx) induce coordinated upregulation of JNK1 with c-Fos, c-Jun, MEKK1, and p38. Through these sophisticated regulatory mechanisms, JNK1/3 (F-5) antibody serves as a valuable tool for investigating stress responses, cancer biology, and immune system regulation.

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-JNK1/3 Antibody (F-5) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-JNK1/3 Antibody (F-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

JNK1/3 Antibody (F-5) Specifications

Alternate Product Name: JNK1/3

Product Clone Name: F-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 364-385

Epitope Target: JNK1 p46

Epitope Species: human

Product Reactivity/Detection: all JNK1 and JNK3 p46 and p54 isoforms

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for JNK1/3 Antibody (F-5): P: Blocking Peptide

The preferred p-JNK antibody is p-JNK Antibody (G-7): sc-6254.