JNK1/3 Antibody (F-5) recommended for detection of all JNK1 and JNK3 p46 and p54 isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

JNK1/3 Antibody (F-5): sc-514539

JNK1/3 Antibody (F-5) is rated 3.5 out of 5 by 2.
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Datasheets

    • Anti-JNK1/3 Antibody (F-5) is a mouse monoclonal IgG3 (kappa light chain) JNK1/3 antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 364-385 near the C-terminus of JNK1 p46 of human origin
    • recommended for detection of all JNK1 and JNK3 p46 and p54 isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • blocking peptide, sc-514539 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See JNK (D-2): sc-7345 for JNK1/3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of JNK1/3 (F-5): sc-514539.

What application is the blocking peptide sc-514539 P appropriate for?

Asked by: Trav11
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 3 out of 5 by from Not very good I had tested it in C42B and VCaP cell lines. I got a lot of bands and I don't know which one it is.
Date published: 2017-10-11
Rated 4 out of 5 by from worked In A549 cells 46kD JNK1 was detected. 54 kD was not. The detected band was decreased by siJNK1 transfection. There are no non-specific band. 1/1000 dil in 2% BSA TBST, o/n reaction.
Date published: 2017-03-24
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