JNK1 Antibody (D-6): sc-137018

JNK1 Antibody (D-6) is a mouse monoclonal IgG2b kappa light chain antibody raised against full-length human JNK1 p46 (amino acids 1-384), a crucial stress-activated protein kinase. JNK1 mouse monoclonal antibody (D-6) recognizes an epitope within the immunogen sequence and demonstrates broad species cross-reactivity, detecting both p46 and p54 isoforms of JNK1 in mouse, rat, and human samples through Western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA) applications. As a member of the mitogen-activated protein kinase (MAPK) family, JNK1 orchestrates sophisticated cellular responses to stress, inflammation, and apoptotic signals. JNK1′s activation mechanism involves precise dual phosphorylation at two specific residues: threonine 183 and tyrosine 185. These post-translational modifications prove essential for JNK1′s kinase activity, enabling effective responses to environmental stresses and cytokines. JNK1′s significance extends to immune system regulation, where JNK1 plays a vital role in the activation and differentiation of CD4+ helper T cells into TH1 and TH2 effector cells. Recent studies have revealed complex regulation of JNK1 activity by various stimuli: capsaicin triggers JNK1 and p38 activation in ras-transformed human breast epithelial cells, while nitrogen oxides (NOx) induce upregulation of JNK1 alongside other signaling components including c-Fos, c-Jun, MEKK1, and p38. Through phosphorylation of c-Jun, JNK1 influences fundamental cellular processes including growth, differentiation, and survival. The dysregulation of these JNK1-mediated pathways contributes to various pathological conditions, including cancer and neurodegenerative diseases, making JNK1 an important therapeutic target. Through these diverse cellular functions, JNK1 (D-6) antibody serves as a valuable tool for investigating both normal signaling mechanisms and disease pathways.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-JNK1 Antibody (D-6) has 24 citations in a variety of scientific publications.

Primary antibodies like Anti-JNK1 Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

JNK1 Antibody (D-6) Specifications

Alternate Product Name: JNK1

Product Clone Name: D-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-384

Epitope Target: JNK1 p46

Epitope Species: human

Product Reactivity/Detection: JNK1 p46 and p54 isoforms

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 24 citations in various scientific publications

Available Conjugates/Forms for JNK1 Antibody (D-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred p-JNK antibody is p-JNK Antibody (G-7): sc-6254.