JNK Antibody (D-2): sc-7345

JNK Antibody (D-2) is a mouse monoclonal IgG1 kappa light chain antibody raised against full-length human JNK2 (amino acids 1-424), a crucial stress-activated protein kinase. JNK mouse monoclonal antibody (D-2) recognizes an epitope within the JNK immunogen sequence and demonstrates broad species cross-reactivity, detecting JNK proteins in mouse, rat, and human samples through Western blotting (WB), immunoprecipitation (IP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA) applications. Anti-JNK monoclonal antibody (D-2) is available in non-conjugated form and multiple conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, offering versatility for diverse experimental needs. The JNK proteins, also known as MAPK8/9/10 or SAPK/JNK, function as serine/threonine kinases that respond to stress stimuli, cytokines, and growth factors by phosphorylating and activating transcription factors including c-Jun, ATF2, and Elk-1. Three distinct genes (JNK1, JNK2, and JNK3) produce ten different isoforms through alternative mRNA splicing, including p46 and p54 variants, each contributing to the regulation of cellular processes such as proliferation, differentiation, apoptosis, and stress responses. JNK participates in sophisticated protein networks, forming complexes with scaffold proteins like JIP (JNK-interacting proteins), Bcl-2 family members involved in apoptosis regulation, and other signaling kinases such as MEKK1 and p38 MAPK. These interactions create intricate signaling cascades that influence cell growth and transformation. When JNK signaling becomes dysregulated, the resulting disruption can contribute to various diseases including cancer, neurodegenerative disorders, diabetes, and cardiovascular conditions. Through broad reactivity with all JNK1, JNK2, and JNK3 p46 and p54 isoforms, JNK (D-2) antibody serves as a valuable tool for investigating these complex signaling networks and their roles in both normal cellular functions and disease states.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

JNK Antibody (D-2) References:

1. JNK regulation of hepatic manifestations of the metabolic syndrome.  |  Czaja, MJ. 2010. Trends Endocrinol Metab. 21: 707-13. PMID: 20888782
2. JNK Signaling: Regulation and Functions Based on Complex Protein-Protein Partnerships.  |  Zeke, A., et al. 2016. Microbiol Mol Biol Rev. 80: 793-835. PMID: 27466283
3. JNK signaling in cancer cell survival.  |  Wu, Q., et al. 2019. Med Res Rev. 39: 2082-2104. PMID: 30912203
4. JNK Signaling Pathway Involvement in Spinal Cord Neuron Development and Death.  |  Schellino, R., et al. 2019. Cells. 8: PMID: 31817379
5. Selective inhibitors for JNK signalling: a potential targeted therapy in cancer.  |  Wu, Q., et al. 2020. J Enzyme Inhib Med Chem. 35: 574-583. PMID: 31994958
6. The JNK Signaling Pathway in Inflammatory Skin Disorders and Cancer.  |  Hammouda, MB., et al. 2020. Cells. 9: PMID: 32252279
7. Slik maintains tissue homeostasis by preventing JNK-mediated apoptosis.  |  Li, C., et al. 2023. Cell Div. 18: 16. PMID: 37794497
8. JNK regulates ciliogenesis through the interflagellar transport complex and actin networks.  |  Chatzifrangkeskou, M., et al. 2023. J Cell Biol. 222: PMID: 37851005
9. The Role of the Dysregulated JNK Signaling Pathway in the Pathogenesis of Human Diseases and Its Potential Therapeutic Strategies: A Comprehensive Review.  |  Yan, H., et al. 2024. Biomolecules. 14: PMID: 38397480

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-JNK Antibody (D-2) has 705 citations in a variety of scientific publications.

Primary antibodies like Anti-JNK Antibody (D-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

JNK Antibody (D-2) Specifications

Alternate Product Name: JNK

Product Clone Name: D-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-424

Epitope Target: JNK2 p54

Epitope Species: human

Product Reactivity/Detection: all JNK1, JNK2 and JNK3 p46 and p54 isoforms

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 705 citations in various scientific publications

Available Conjugates/Forms for JNK Antibody (D-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred p-JNK antibody is p-JNK Antibody (G-7): sc-6254.