Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
JMJD2A CRISPR/Cas9 KO Plasmid (m): sc-432966
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- JMJD2A CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the JMJD2A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: JMJD2A Antibody (D-9): sc-271210
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
JMJD2A CRISPR/Cas9 KO Plasmid (m) | sc-432966 | 20 µg | $397.00 |
Overview
Kdm4a encodes the mouse histone lysine demethylase JMJD2A (KDM4A), a JmjC-domain enzyme that removes repressive and activating methyl marks on H3K9 and H3K36 to shape chromatin accessibility and transcriptional programs. By coupling epigenetic state to DNA-templated processes, JMJD2A influences cell-cycle progression, replication stress responses, and genome stability, and it interfaces with pathways regulating differentiation and hormone-responsive transcription. Dysregulated KDM4-family activity has been linked to altered oncogenic signaling, defective DNA repair, and aberrant developmental gene expression, making Kdm4a a common target in studies of chromatin-driven disease mechanisms. In mouse systems, Kdm4a perturbation is frequently used to interrogate how histone methylation dynamics coordinate transcriptional networks and cellular plasticity.
JMJD2A CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Kdm4a gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Kdm4a together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Kdm4a open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish JMJD2A protein expression.
This CRISPR knockout system enables efficient generation of Kdm4a-deficient cell models for investigation of JMJD2A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Kdm4a exon(s) critical for JMJD2A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Kdm4a genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by JMJD2A CRISPR/Cas9 KO Plasmid (m) and JMJD2A CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Kdm4a locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by JMJD2A HDR Plasmid (m) and JMJD2A HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Kdm4a homology arms to support homology-directed repair at defined Kdm4a target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.