JIP-4 Antibody (H-8): sc-271492
- JIP-4 Antibody (H-8) is a mouse monoclonal IgG1 κ JIP-4 antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 164-328 mapping near the N-terminus of JIP-4 of human origin
- JIP-4 Antibody (H-8) is recommended for detection of JIP-4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-JIP-4 Antibody (H-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for JIP-4 Antibody (H-8) for WB and IHC(P) applications. This reagent is now offered in a bundle with JIP-4 Antibody (H-8) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
JIP-4 Antibody (H-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects JIP-4 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). JIP-4 (H-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. JIP-4, also known as C-jun-amino-terminal kinase-interacting protein 4, is a 1,321 amino acid protein encoded by the human gene SPAG9. JIP-4 plays a crucial role in cellular signaling by acting as a scaffold that selectively mediates c-Jun N-terminal kinase (JNK) signaling, which is vital for various cellular processes, including stress responses, apoptosis, and differentiation. JIP-4 is primarily located in the cytoplasm and perinuclear region, where JIP-4 aggregates specific components of the mitogen-activated protein kinase (MAPK) cascade to form functional signaling modules. This localization allows for precise regulation of JNK signaling pathways, which can influence cell fate decisions in response to external stimuli. JIP-4 has eight known isoforms, with expression levels that vary depending on tissue type and disease state, highlighting JIP-4′s potential as a biomarker for various conditions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
JIP-4 Antibody (H-8) References:
- Kinesin-dependent axonal transport is mediated by the sunday driver (SYD) protein. | Bowman, AB., et al. 2000. Cell. 103: 583-94. PMID: 11106729
- JLP: A scaffolding protein that tethers JNK/p38MAPK signaling modules and transcription factors. | Lee, CM., et al. 2002. Proc Natl Acad Sci U S A. 99: 14189-94. PMID: 12391307
- A novel protein highly expressed in testis is overexpressed in systemic sclerosis fibroblasts and targeted by autoantibodies. | Yasuoka, H., et al. 2003. J Immunol. 171: 6883-90. PMID: 14662895
- Characterization of a novel human sperm-associated antigen 9 (SPAG9) having structural homology with c-Jun N-terminal kinase-interacting protein. | Jagadish, N., et al. 2005. Biochem J. 389: 73-82. PMID: 15693750
- Sperm associated antigen 9 (SPAG9): a new member of c-Jun NH2 -terminal kinase (JNK) interacting protein exclusively expressed in testis. | Jagadish, N., et al. 2005. Keio J Med. 54: 66-71. PMID: 16077255
- Direct interaction of the molecular scaffolds POSH and JIP is required for apoptotic activation of JNKs. | Kukekov, NV., et al. 2006. J Biol Chem. 281: 15517-24. PMID: 16571722
- ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. | Matsuoka, S., et al. 2007. Science. 316: 1160-6. PMID: 17525332
- Cloning of a novel human testis mRNA specifically expressed in testicular haploid germ cells, having unique palindromic sequences and encoding a leucine zipper dimerization motif. | Shankar, S., et al. 1998. Biochem Biophys Res Commun. 243: 561-5. PMID: 9480848
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
JIP-4 Antibody (H-8) | sc-271492 | 200 µg/ml | $322.00 | |||
JIP-4 Antibody (H-8): m-IgGκ BP-HRP Bundle | sc-521958 | 200 µg Ab, 40 µg BP | $361.00 | |||
JIP-4 Antibody (H-8) AC | sc-271492 AC | 500 µg/ml, 25% agarose | $424.00 | |||
JIP-4 Antibody (H-8) HRP | sc-271492 HRP | 200 µg/ml | $322.00 | |||
JIP-4 Antibody (H-8) FITC | sc-271492 FITC | 200 µg/ml | $336.00 | |||
JIP-4 Antibody (H-8) PE | sc-271492 PE | 200 µg/ml | $349.00 | |||
JIP-4 Antibody (H-8) Alexa Fluor® 488 | sc-271492 AF488 | 200 µg/ml | $364.00 | |||
JIP-4 Antibody (H-8) Alexa Fluor® 546 | sc-271492 AF546 | 200 µg/ml | $364.00 | |||
JIP-4 Antibody (H-8) Alexa Fluor® 594 | sc-271492 AF594 | 200 µg/ml | $364.00 | |||
JIP-4 Antibody (H-8) Alexa Fluor® 647 | sc-271492 AF647 | 200 µg/ml | $364.00 | |||
JIP-4 Antibody (H-8) Alexa Fluor® 680 | sc-271492 AF680 | 200 µg/ml | $364.00 | |||
JIP-4 Antibody (H-8) Alexa Fluor® 790 | sc-271492 AF790 | 200 µg/ml | $364.00 |