JIK Antibody (C-3): sc-377083
- JIK Antibody (C-3) is a mouse monoclonal IgG1 κ JIK antibody provided at 200 µg/ml
- raised against amino acids 367-426 mapping within an internal region of JIK of human origin
- JIK Antibody (C-3) is recommended for detection of JIK of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-JIK Antibody (C-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for JIK Antibody (C-3) for WB and IHC(P) applications. This reagent is now offered in a bundle with JIK Antibody (C-3) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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JIK Antibody (C-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects JIK protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). JIK (C-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. JNK/SAPK-inhibitory kinase (JIK) is a serine/threonine kinase that plays a crucial role in cellular signaling pathways by inhibiting c-Jun NH2-terminal kinase/stress-activated protein kinase (JNK/SAPK), which is activated in response to various cellular stresses and extracellular signals. This inhibition is vital as JNK/SAPK is involved in regulating critical processes such as cell survival, apoptosis, and proliferation, particularly during mouse development. JIK includes a kinase domain closely related to the GCK-like subfamily of STE20 kinases, while its non-catalytic domain shares similarities with a putative serine/threonine kinase from Caenorhabditis elegans known as SULU. JIK is negatively regulated by epidermal growth factor (EGF) and tyrosine kinase receptors, and JIK localization varies depending on the cellular context; in unstimulated human T cells, JIK is predominantly cytoplasmic, whereas in continuously dividing human T cells, such as those found in Jurkat lymphoma, JIK translocates to the nucleus, highlighting its dynamic role in cellular signaling and function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
JIK Antibody (C-3) References:
- Human JIK, a novel member of the STE20 kinase family that inhibits JNK and is negatively regulated by epidermal growth factor. | Tassi, E., et al. 1999. J Biol Chem. 274: 33287-95. PMID: 10559204
- Cloning of DPK, a novel dendritic cell-derived protein kinase activating the ERK1/ERK2 and JNK/SAPK pathways. | Zhang, W., et al. 2000. Biochem Biophys Res Commun. 274: 872-9. PMID: 10924369
- Activation of caspase-12, an endoplastic reticulum (ER) resident caspase, through tumor necrosis factor receptor-associated factor 2-dependent mechanism in response to the ER stress. | Yoneda, T., et al. 2001. J Biol Chem. 276: 13935-40. PMID: 11278723
- Comparative studies of a new subfamily of human Ste20-like kinases: homodimerization, subcellular localization, and selective activation of MKK3 and p38. | Yustein, JT., et al. 2003. Oncogene. 22: 6129-41. PMID: 13679851
- SEREX identification of new tumour-associated antigens in cutaneous T-cell lymphoma. | Hartmann, TB., et al. 2004. Br J Dermatol. 150: 252-8. PMID: 14996095
- Physiological roles of SAPK/JNK signaling pathway. | Nishina, H., et al. 2004. J Biochem. 136: 123-6. PMID: 15496581
- Sensitized RNAi screen of human kinases and phosphatases identifies new regulators of apoptosis and chemoresistance. | MacKeigan, JP., et al. 2005. Nat Cell Biol. 7: 591-600. PMID: 15864305
- JNK inhibitory kinase is up-regulated in retinal ganglion cells after axotomy and enhances BimEL expression level in neuronal cells. | Wakabayashi, T., et al. 2005. J Neurochem. 95: 526-36. PMID: 16092929
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
JIK Antibody (C-3) | sc-377083 | 200 µg/ml | $322.00 | |||
JIK Antibody (C-3): m-IgGκ BP-HRP Bundle | sc-523179 | 200 µg Ab, 40 µg BP | $361.00 | |||
JIK Antibody (C-3) AC | sc-377083 AC | 500 µg/ml, 25% agarose | $424.00 | |||
JIK Antibody (C-3) HRP | sc-377083 HRP | 200 µg/ml | $322.00 | |||
JIK Antibody (C-3) FITC | sc-377083 FITC | 200 µg/ml | $336.00 | |||
JIK Antibody (C-3) PE | sc-377083 PE | 200 µg/ml | $349.00 | |||
JIK Antibody (C-3) Alexa Fluor® 488 | sc-377083 AF488 | 200 µg/ml | $364.00 | |||
JIK Antibody (C-3) Alexa Fluor® 546 | sc-377083 AF546 | 200 µg/ml | $364.00 | |||
JIK Antibody (C-3) Alexa Fluor® 594 | sc-377083 AF594 | 200 µg/ml | $364.00 | |||
JIK Antibody (C-3) Alexa Fluor® 647 | sc-377083 AF647 | 200 µg/ml | $364.00 | |||
JIK Antibody (C-3) Alexa Fluor® 680 | sc-377083 AF680 | 200 µg/ml | $364.00 | |||
JIK Antibody (C-3) Alexa Fluor® 790 | sc-377083 AF790 | 200 µg/ml | $364.00 |