Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Jagged1 CRISPR/Cas9 KO Plasmid (m): sc-421197
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Jagged1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Jagged1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Jagged1 Antibody (E-12): sc-390177
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Jagged1 CRISPR/Cas9 KO Plasmid (m) | sc-421197 | 20 µg | $397.00 |
Overview
Jag1 encodes the membrane-bound ligand Jagged1, a canonical activator of Notch receptors that regulates juxtacrine signaling controlling cell fate decisions, boundary formation, and tissue patterning in the mouse. Jagged1–Notch engagement triggers proteolytic release of the Notch intracellular domain and transcriptional reprogramming via RBPJ/CSL complexes, shaping processes such as angiogenesis, stem and progenitor maintenance, and epithelial–mesenchymal interactions. In developmental and disease models, altered Jag1/Notch signaling is linked to dysregulated differentiation, aberrant vascular remodeling, and changes in immune and stromal cell behavior. Because Notch output is highly context dependent, Jag1 loss-of-function provides a direct way to dissect ligand-specific signaling thresholds and pathway crosstalk in vivo and in cultured cells.
Jagged1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Jag1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Jag1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Jag1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Jagged1 protein expression.
This CRISPR knockout system enables efficient generation of Jag1-deficient cell models for investigation of Jagged1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Jag1 exon(s) critical for Jagged1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Jag1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Jagged1 CRISPR/Cas9 KO Plasmid (m) and Jagged1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Jag1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Jagged1 HDR Plasmid (m) and Jagged1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Jag1 homology arms to support homology-directed repair at defined Jag1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.