ITM1 Antibody (JA-07): sc-100796

ITM1 Antibody (JA-07) is a mouse monoclonal IgG1 antibody that detects ITM1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). ITM1, also known as integral membrane protein 1 or transmembrane protein TMC, is a crucial multi-pass membrane protein predominantly expressed in the liver, pancreas, muscle, placenta, and skin fibroblasts, where ITM1 localizes to the endoplasmic reticulum membrane. Anti-ITM1 antibody (JA-07) recognizes a protein that plays a vital role in the oligosaccharyltransferase (OST) complex, which is essential for the glycosylation of nascent proteins. Specifically, ITM1 catalyzes the transfer of high mannose oligosaccharides to asparagine residues in proteins entering the ER, a process critical for proper protein folding and stability. The glycosylation process facilitated by ITM1 is not only important for protein function but also influences cell signaling and interactions, making ITM1 a key player in various biological processes. Compared to its homolog SIMP, ITM1 exhibits lower activity but greater substrate selectivity, highlighting ITM1′s specialized role in the glycosylation pathway. With consistent detection across multiple species and applications, ITM1 monoclonal antibody (JA-07) serves as an invaluable tool for researchers studying protein glycosylation and its implications in cellular functions.

The preferred ITM1 antibody is ITM1 Antibody (A-2): sc-390227.