Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ITF-2 CRISPR/Cas9 KO Plasmid (h): sc-402815
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ITF-2/TCF4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ITF-2/TCF4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ITF-2/TCF4 Antibody (C-8): sc-393407
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ITF-2 CRISPR/Cas9 KO Plasmid (h) | sc-402815 | 20 µg | $397.00 |
Overview
TCF4 encodes the basic helix-loop-helix transcription factor ITF-2 (also known as E2-2), a DNA-binding regulator that modulates lineage-specific gene expression programs through E-box motif recognition and dimerization with other bHLH proteins. ITF-2 participates in transcriptional networks governing cellular differentiation, proliferation, and developmental patterning, and it intersects with pathways that shape cell fate decisions and chromatin-dependent gene regulation. Dysregulated TCF4 activity has been linked to neurodevelopmental biology and immune cell specification, and altered transcriptional control by ITF-2 is associated with disease-relevant phenotypes including developmental disorders and malignancy-associated transcriptional rewiring. As a context-dependent regulator, TCF4 is frequently studied for its role in controlling gene expression dynamics, enhancer activity, and downstream pathway outputs across diverse human cell models.
ITF-2/TCF4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TCF4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TCF4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TCF4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ITF-2/TCF4 protein expression.
This CRISPR knockout system enables efficient generation of TCF4-deficient cell models for investigation of ITF-2/TCF4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TCF4 exon(s) critical for ITF-2/TCF4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TCF4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ITF-2/TCF4 CRISPR/Cas9 KO Plasmid (h) and ITF-2/TCF4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TCF4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ITF-2/TCF4 HDR Plasmid (h) and ITF-2/TCF4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TCF4 homology arms to support homology-directed repair at defined TCF4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.