ISG15 (m): 293T Lysate: sc-121117

ISG15 (m): 293T Lysate, derived from the HEK 293T cell line, is pivotal for the study of the ISG15 protein, a ubiquitin-like modifier in murine systems, focusing on its role in cellular signaling and protein modification within research environments devoid of clinical implications. The 293T cell line, a human embryonic kidney cell derivative, has been engineered to express the SV40 large T antigen, which significantly enhances the replication and expression of episomal plasmids, thus facilitating the overexpression of proteins such as ISG15. This lysate is extensively used to explore the process of ISGylation, where ISG15 is conjugated to target proteins, affecting their stability and function. Researchers employ techniques such as immunoprecipitation, mass spectrometry, and Western blotting to investigate how ISG15 influences signaling pathways and cellular stress responses, particularly how it modulates the antiviral state in cells. By studying ISG15 in the 293T context, scientists can dissect its role in post-translational modifications and understand its regulatory mechanisms on a molecular level, offering insights into basic biological processes and cellular defense mechanisms.