IscU1/2 Antibody (B-3): sc-271536

IscU1/2 Antibody (B-3) is a mouse monoclonal IgG2a antibody that detects IscU1/2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IscU1 and IscU2 play a crucial role in the biogenesis of iron-sulfur (Fe-S) clusters, which are essential cofactors involved in various cellular processes, including electron transfer in oxidative phosphorylation and enzymatic reactions in key metabolic pathways. The localization of IscU proteins is particularly important, as IscU1 is primarily found in the cytosol while IscU2 is localized in the mitochondria, allowing for the assembly of Fe-S clusters in multiple cellular compartments. This spatial distribution is vital for maintaining cellular iron homeostasis and preventing oxidative damage, especially in cells lacking cytosolic copper/zinc superoxide dismutase. Furthermore, IscU proteins interact with IscS, a cysteine desulfurase, to facilitate the sequestration of inorganic sulfur necessary for Fe-S cluster assembly, highlighting their collaborative role in mitochondrial and cytosolic functions. Anti-IscU1/2 antibody (B-3) is an invaluable tool for researchers studying the dynamics of Fe-S cluster biogenesis and its implications in cellular metabolism and oxidative stress responses.

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IscU1/2 Antibody (B-3) References:

1. Subcellular compartmentalization of human Nfu, an iron-sulfur cluster scaffold protein, and its ability to assemble a [4Fe-4S] cluster.  |  Tong, WH., et al. 2003. Proc Natl Acad Sci U S A. 100: 9762-7. PMID: 12886008
2. The Arabidopsis chloroplastic NifU-like protein CnfU, which can act as an iron-sulfur cluster scaffold protein, is required for biogenesis of ferredoxin and photosystem I.  |  Yabe, T., et al. 2004. Plant Cell. 16: 993-1007. PMID: 15031412
3. Extra-mitochondrial localisation of frataxin and its association with IscU1 during enterocyte-like differentiation of the human colon adenocarcinoma cell line Caco-2.  |  Acquaviva, F., et al. 2005. J Cell Sci. 118: 3917-24. PMID: 16091420
4. Splice mutation in the iron-sulfur cluster scaffold protein ISCU causes myopathy with exercise intolerance.  |  Mochel, F., et al. 2008. Am J Hum Genet. 82: 652-60. PMID: 18304497
5. Specialized Hsp70 chaperone (HscA) binds preferentially to the disordered form, whereas J-protein (HscB) binds preferentially to the structured form of the iron-sulfur cluster scaffold protein (IscU).  |  Kim, JH., et al. 2012. J Biol Chem. 287: 31406-13. PMID: 22782893
6. Human mitochondrial chaperone (mtHSP70) and cysteine desulfurase (NFS1) bind preferentially to the disordered conformation, whereas co-chaperone (HSC20) binds to the structured conformation of the iron-sulfur cluster scaffold protein (ISCU).  |  Cai, K., et al. 2013. J Biol Chem. 288: 28755-70. PMID: 23940031
7. The presence of multiple cellular defects associated with a novel G50E iron-sulfur cluster scaffold protein (ISCU) mutation leads to development of mitochondrial myopathy.  |  Saha, PP., et al. 2014. J Biol Chem. 289: 10359-10377. PMID: 24573684
8. Overlapping binding sites of the frataxin homologue assembly factor and the heat shock protein 70 transfer factor on the Isu iron-sulfur cluster scaffold protein.  |  Manicki, M., et al. 2014. J Biol Chem. 289: 30268-30278. PMID: 25228696
9. New insights into the pathology of pulmonary hypertension: implication of the miR-210/ISCU1/2/Fe-S axis.  |  Tang, H., et al. 2015. EMBO Mol Med. 7: 689-91. PMID: 25851536
10. The Human Iron-Sulfur Assembly Complex Catalyzes the Synthesis of [2Fe-2S] Clusters on ISCU2 That Can Be Transferred to Acceptor Molecules.  |  Fox, NG., et al. 2015. Biochemistry. 54: 3871-9. PMID: 26016389

The preferred IscU1/2 antibody is IscU1/2 Antibody (B-12): sc-271468.