ISCA2 CRISPR/Cas9 KO Plasmid (h): sc-406334
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ISCA2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ISCA2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ISCA2 CRISPR/Cas9 KO Plasmid (h) | sc-406334 | 20 µg | $397.00 |
Overview
ISCA2 encodes a conserved mitochondrial protein required for maturation and trafficking of iron–sulfur (Fe–S) clusters, supporting the assembly of Fe–S-dependent enzymes involved in oxidative phosphorylation, mitochondrial translation, and redox homeostasis. Through its role in the late-acting ISC pathway, ISCA2 helps maintain respiratory chain function and protects cells from metabolic stress linked to impaired electron transport. Disruption of ISCA2 has been associated with mitochondrial dysfunction and neurodegenerative phenotypes consistent with defective Fe–S cluster biogenesis. As a result, ISCA2 is frequently studied in the context of mitochondrial quality control, energy metabolism, and pathways that couple iron handling to genome stability and cellular stress responses.
ISCA2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ISCA2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ISCA2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ISCA2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ISCA2 protein expression.
This CRISPR knockout system enables efficient generation of ISCA2-deficient cell models for investigation of ISCA2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ISCA2 exon(s) critical for ISCA2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ISCA2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ISCA2 CRISPR/Cas9 KO Plasmid (h) and ISCA2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ISCA2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ISCA2 HDR Plasmid (h) and ISCA2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ISCA2 homology arms to support homology-directed repair at defined ISCA2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.