Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IRSp53 Double Nickase Plasmid (h): sc-403072-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IRSp53 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IRSp53 Double Nickase Plasmid (h) and IRSp53 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BAIAP2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IRSp53 Antibody (46): sc-136470
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IRSp53 Double Nickase Plasmid (h) | sc-403072-NIC | 20 µg | $410.00 |
BAIAP2 encodes IRSp53, an I-BAR domain–containing adaptor that links membrane curvature sensing to actin cytoskeleton remodeling by coordinating Rho-family GTPase signaling and actin regulators such as WAVE and Mena/VASP. IRSp53 supports filopodia and lamellipodia formation, endocytosis, and growth factor–dependent pathways that control cell migration, adhesion, and neurite outgrowth. Through these functions, BAIAP2 is commonly studied in contexts where cytoskeletal dynamics and membrane trafficking shape cellular behavior, including neurodevelopmental and synaptic processes. Dysregulation of IRSp53-associated networks has been investigated in relation to altered cell motility and invasion phenotypes as well as neurological disease biology.
IRSp53 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BAIAP2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BAIAP2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BAIAP2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BAIAP2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.