IRF-5 Antibody (10T1): sc-56714

IRF-5 Antibody (10T1) is a mouse monoclonal IgG1 antibody that detects IRF-5 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IRF-5 (10T1) antibody is available in a non-conjugated form, allowing for versatile experimental use. Interferon regulatory factor 5 (IRF-5) is a crucial member of the IRF family of DNA-binding proteins, which also includes IRF-1, IRF-2, IRF-3, IRF-4, IRF-6, IRF-7, ISGF-3γ p48, and interferon consensus sequence-binding protein (ICSBP). This family plays a significant role in regulating both interferon and interferon-inducible genes, making IRF-5 essential for immune response modulation. Notably, IRF-5 is involved in the signaling pathways activated by viral infections, directly transducing signals that lead to the expression of various cytokines and chemokines, which are vital for orchestrating the immune response. Additionally, IRF-5 is known to interact with several specific proteins, including p53, which, while a direct target of IRF-5, does not influence cell cycle regulatory and pro-apoptotic functions, highlighting the complex regulatory mechanisms at play in immune signaling and cellular responses.

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IRF-5 Antibody (10T1) References:

1. Interferon regulatory factors: the next generation.  |  Mamane, Y., et al. 1999. Gene. 237: 1-14. PMID: 10524230
2. Virus-specific activation of a novel interferon regulatory factor, IRF-5, results in the induction of distinct interferon alpha genes.  |  Barnes, BJ., et al. 2001. J Biol Chem. 276: 23382-90. PMID: 11303025
3. Identification of the interferon regulatory factor 5 gene (IRF-5) as a direct target for p53.  |  Mori, T., et al. 2002. Oncogene. 21: 2914-8. PMID: 11973653
4. Multiple regulatory domains of IRF-5 control activation, cellular localization, and induction of chemokines that mediate recruitment of T lymphocytes.  |  Barnes, BJ., et al. 2002. Mol Cell Biol. 22: 5721-40. PMID: 12138184
5. Virus-induced heterodimer formation between IRF-5 and IRF-7 modulates assembly of the IFNA enhanceosome in vivo and transcriptional activity of IFNA genes.  |  Barnes, BJ., et al. 2003. J Biol Chem. 278: 16630-41. PMID: 12600985
6. Interferon regulatory factor 5, a novel mediator of cell cycle arrest and cell death.  |  Barnes, BJ., et al. 2003. Cancer Res. 63: 6424-31. PMID: 14559832
7. Unique contribution of IRF-5-Ikaros axis to the B-cell IgG2a response.  |  Fang, CM., et al. 2012. Genes Immun. 13: 421-30. PMID: 22535200
8. IRF-5 and NF-κB p50 co-regulate IFN-β and IL-6 expression in TLR9-stimulated human plasmacytoid dendritic cells.  |  Steinhagen, F., et al. 2013. Eur J Immunol. 43: 1896-906. PMID: 23616277
9. Constitutive expression of IRF-5 in HTLV-1-infected T cells.  |  Ishikawa, C., et al. 2015. Int J Oncol. 47: 361-9. PMID: 26004104
10. IRF-5 Promotes Cell Death in CD4 T Cells during Chronic Infection.  |  Fabié, A., et al. 2018. Cell Rep. 24: 1163-1175. PMID: 30067973
11. The TLR7/IRF-5 axis sensitizes memory CD4+ T cells to Fas-mediated apoptosis during HIV-1 infection.  |  Carmona-Pérez, L., et al. 2023. JCI Insight. 8: PMID: 37227774
12. Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins.  |  Darnell, JE., et al. 1994. Science. 264: 1415-21. PMID: 8197455

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IRF-5 Antibody (10T1) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-IRF-5 Antibody (10T1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IRF-5 Antibody (10T1) Specifications

Alternate Product Name: IRF-5

Product Clone Name: 10T1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 176-240

Epitope Target: IRF-5

Epitope Species: human

Product Reactivity/Detection: IRF-5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 50 µg IgG₁ in 0.5 ml of PBS with < 0.1% sodium azide, 0.1% gelatin and 1% glycerol

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for IRF-5 Antibody (10T1): N/A