IRF3 Antibody (SL-12): sc-33641

IRF-3 Antibody (SL-12) is a mouse monoclonal IgG1 kappa light chain antibody designed to target the Interferon Regulatory Factor 3 (IRF-3) protein with exceptional precision. Engineered against a recombinant IRF-3 fusion protein corresponding to human IRF-3 (amino acids 56-427), anti-IRF-3 antibody (SL-12) reliably detects IRF-3 from mouse, rat, and human species across a variety of applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). IRF-3, also referred to as IIAE7, is a critical transcription factor in the innate immune response, playing a vital role in the detection of viral infections and the subsequent activation of type I interferons, such as IFN-β. Upon viral infection, IRF-3 undergoes phosphorylation, dimerizes, and translocates to the nucleus where IRF-3 binds to specific DNA elements to initiate the transcription of interferon genes, thereby orchestrating a robust antiviral defense mechanism. Understanding and analyzing IRF-3′s function is essential for elucidating the molecular pathways involved in immune regulation and the body′s response to pathogens. IRF-3 (SL-12) monoclonal antibody is available in both non-conjugated form and a variety of conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatile tools for researchers to investigate the intricate roles of IRF-3 in cellular processes and disease states.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

IRF3 Antibody (SL-12) References:

1. Triggering the interferon response: the role of IRF-3 transcription factor.  |  Hiscott, J., et al. 1999. J Interferon Cytokine Res. 19: 1-13. PMID: 10048763
2. Overlapping and distinct mechanisms regulating IRF-3 and IRF-7 function.  |  Servant, MJ., et al. 2002. J Interferon Cytokine Res. 22: 49-58. PMID: 11846975
3. Control of IRF-3 activation by phosphorylation.  |  Yoneyama, M., et al. 2002. J Interferon Cytokine Res. 22: 73-6. PMID: 11846977
4. Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3.  |  Seth, RB., et al. 2005. Cell. 122: 669-82. PMID: 16125763
5. The kinase complex responsible for IRF-3-mediated IFN-beta production in myeloid dendritic cells (mDC).  |  Sasai, M., et al. 2006. J Biochem. 139: 171-5. PMID: 16452304
6. Distinct functions of IRF-3 and IRF-7 in IFN-alpha gene regulation and control of anti-tumor activity in primary macrophages.  |  Solis, M., et al. 2006. Biochem Pharmacol. 72: 1469-76. PMID: 16846591
7. IRF-3-dependent and augmented target genes during viral infection.  |  Andersen, J., et al. 2008. Genes Immun. 9: 168-75. PMID: 18094709
8. The roles of IRF-3 and IRF-7 in innate antiviral immunity against dengue virus.  |  Chen, HW., et al. 2013. J Immunol. 191: 4194-201. PMID: 24043884
9. Thymoquinone Suppresses IRF-3-Mediated Expression of Type I Interferons via Suppression of TBK1.  |  Aziz, N., et al. 2018. Int J Mol Sci. 19: PMID: 29751576
10. The Structural Basis of IRF-3 Activation upon Phosphorylation.  |  Jing, T., et al. 2020. J Immunol. 205: 1886-1896. PMID: 32826280

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IRF3 Antibody (SL-12) has 86 citations in a variety of scientific publications.

Primary antibodies like Anti-IRF3 Antibody (SL-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IRF3 Antibody (SL-12) Specifications

Alternate Product Name: IRF-3

Product Clone Name: SL-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 56-427

Epitope Target: IRF-3

Epitope Species: human

Product Reactivity/Detection: IRF-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 86 citations in various scientific publications

Available Conjugates/Forms for IRF3 Antibody (SL-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred IRF3 antibody is IRF3 Antibody (D-3): sc-376455.