IRF-2BP1 Antibody (D-5) recommended for detection of IRF-2BP1 of human origin by WB, IP, IF and ELISA

IRF-2BP1 Antibody (D-5): sc-515314

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Datasheets

    • IRF-2BP1 Antibody (D-5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 443-502 mapping within an internal region of IRF-2BP1 of human origin
    • recommended for detection of IRF-2BP1 of human origin by WB, IP, IF and ELISA

How much sample do you recommend loading on the gel for Western blot detection using IRF-2BP1 (D-5): sc-515314?

Asked by: jerojero
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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