Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IRBP CRISPR/Cas9 KO Plasmid (h): sc-403810
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IRBP CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IRBP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IRBP Antibody (A-12): sc-376225
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IRBP CRISPR/Cas9 KO Plasmid (h) | sc-403810 | 20 µg | $397.00 |
Overview
RBP3 encodes interphotoreceptor retinoid-binding protein (IRBP), a large secreted glycoprotein produced by photoreceptors that accumulates in the interphotoreceptor matrix and binds retinoids. IRBP supports visual cycle homeostasis by facilitating the extracellular transport and exchange of 11-cis and all-trans retinoids between photoreceptors and retinal pigment epithelium, contributing to phototransduction recovery and protection from retinoid-mediated oxidative stress. Through these activities, IRBP influences retinoid metabolism, lipid/retinoid trafficking, and extracellular matrix-associated processes critical for retinal function. Altered RBP3/IRBP expression or function has been associated with retinal dysfunction phenotypes and is studied in the context of degenerative and inflammatory eye disease mechanisms.
IRBP CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RBP3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RBP3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RBP3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IRBP protein expression.
This CRISPR knockout system enables efficient generation of RBP3-deficient cell models for investigation of IRBP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RBP3 exon(s) critical for IRBP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RBP3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IRBP CRISPR/Cas9 KO Plasmid (h) and IRBP CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RBP3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IRBP HDR Plasmid (h) and IRBP HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RBP3 homology arms to support homology-directed repair at defined RBP3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.