Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IQGAP3 CRISPR/Cas9 KO Plasmid (h): sc-406335
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IQGAP3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IQGAP3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IQGAP3 Antibody (D-10): sc-393451
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IQGAP3 CRISPR/Cas9 KO Plasmid (h) | sc-406335 | 20 µg | $397.00 |
Overview
IQGAP3 (IQ motif containing GTPase activating protein 3) is a scaffolding protein that coordinates signaling and cytoskeletal dynamics by coupling small GTPases, actin regulatory factors, and kinase cascades. It contributes to cell-cycle progression, directional migration, and adhesion remodeling, integrating inputs from pathways such as MAPK/ERK and Rho-family GTPase signaling to shape proliferative and motility programs. Dysregulated IQGAP3 expression has been reported in multiple tumor contexts and is frequently studied for its association with enhanced proliferation, invasiveness, and altered epithelial–mesenchymal features. As a human gene, IQGAP3 is widely used as a node for dissecting network-level control of cytoskeletal organization and mitogenic signaling in mechanistic disease models.
IQGAP3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the IQGAP3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the IQGAP3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the IQGAP3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IQGAP3 protein expression.
This CRISPR knockout system enables efficient generation of IQGAP3-deficient cell models for investigation of IQGAP3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting IQGAP3 exon(s) critical for IQGAP3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple IQGAP3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IQGAP3 CRISPR/Cas9 KO Plasmid (h) and IQGAP3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the IQGAP3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IQGAP3 HDR Plasmid (h) and IQGAP3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by IQGAP3 homology arms to support homology-directed repair at defined IQGAP3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.