IPP-2 Antibody (70.5): sc-130392
- IPP-2 Antibody (70.5) is a mouse monoclonal IgG2a κ IPP-2 antibody provided at 100 µg/ml
- raised against recombinant IPP-2 of human origin
- recommended for detection of IPP-2 of mouse, rat and human origin by WB, IP, IF and IHC(P)
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for IPP-2 Antibody (70.5). This work is in progress.
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IPP-2 Antibody (70.5) is a mouse monoclonal IgG2a kappa light chain antibody that detects IPP-2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-IPP-2 antibody (70.5) is available as the non-conjugated format. IPP-2, also known as I-2, plays a crucial role in regulating protein phosphatase 1 (PP1) activity, which is essential for various cellular processes, including cell division, metabolism, and signal transduction. Located primarily in the cytosol, IPP-2 interacts with the catalytic subunit of PP1 to form the inactive heterodimer complex PP1I. This complex is vital for maintaining the balance of phosphorylation and dephosphorylation events within the cell. The activation of the PP1I complex is initiated by the reversible phosphorylation of IPP-2 at Thr 72 by glycogen-synthase-kinase (GSK3), which is further enhanced by phosphorylation at Ser 86, Ser 120, and Ser 121 by casein kinase-II. These post-translational modifications are critical as they regulate the activity of the PP1I complex, thereby influencing numerous signaling pathways. Additionally, IPP-2 may function as a chaperone, assisting in the proper folding of PP1, which underscores its importance in cellular homeostasis. The gene encoding human IPP-2 is located on chromosome 6 within the major histocompatibility complex region, highlighting its potential role in immune responses.
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IPP-2 Antibody (70.5) References:
- Separation and characterization of two phosphorylase phosphatase inhibitors from rabbit skeletal muscle. | Huang, FL. and Glinsmann, WH. 1976. Eur J Biochem. 70: 419-26. PMID: 188646
- Identification of the sites on rabbit skeletal muscle protein phosphatase inhibitor-2 phosphorylated by casein kinase-II. | Holmes, CF., et al. 1986. Biochim Biophys Acta. 870: 408-16. PMID: 3008843
- The protein phosphatases of Drosophila melanogaster and their inhibitors. | Orgad, S., et al. 1987. Eur J Biochem. 164: 31-8. PMID: 3030753
- Analysis of the in vivo phosphorylation state of protein phosphatase inhibitor-2 from rabbit skeletal muscle by fast-atom bombardment mass spectrometry. | Holmes, CF., et al. 1987. Biochim Biophys Acta. 929: 208-19. PMID: 3036252
- Identification of protein phosphatases-1 and 2A and inhibitor-2 in oocytes of the starfish Asterias rubens and Marthasterias glacialis. | Pondaven, P. and Cohen, P. 1987. Eur J Biochem. 167: 135-40. PMID: 3040398
- Synergistic phosphorylation and activation of ATP-Mg-dependent phosphoprotein phosphatase by F A/GSK-3 and casein kinase II (PC0.7). | DePaoli-Roach, AA. 1984. J Biol Chem. 259: 12144-52. PMID: 6090457
- Cloning and characterization of human phosphatase inhibitor-2 (IPP-2) sequences. | Sanséau, P., et al. 1994. Mamm Genome. 5: 490-6. PMID: 7949733
- Inhibitor-2 functions like a chaperone to fold three expressed isoforms of mammalian protein phosphatase-1 into a conformation with the specificity and regulatory properties of the native enzyme. | Alessi, DR., et al. 1993. Eur J Biochem. 213: 1055-66. PMID: 8389292
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IPP-2 Antibody (70.5) | sc-130392 | 100 µg/ml | $339.00 |