IPMK CRISPR/Cas9 KO Plasmid (m): sc-427508
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IPMK CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IPMK genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IPMK CRISPR/Cas9 KO Plasmid (m) | sc-427508 | 20 µg | $397.00 |
Overview
Mouse Ipmk encodes inositol polyphosphate multikinase (IPMK), a multifunctional enzyme that phosphorylates inositol phosphates and contributes to the generation of higher-order inositol polyphosphates involved in signal transduction. IPMK interfaces with phosphoinositide and inositol phosphate metabolism, impacting nuclear signaling, transcriptional regulation, and cellular energy homeostasis pathways such as PI3K/AKT and mTOR-associated signaling. Through these roles, IPMK influences processes including growth control, stress responses, and metabolic regulation, and has been studied in contexts where dysregulated inositol phosphate signaling is linked to cancer-associated signaling networks and metabolic disease phenotypes. Its broad pathway connectivity makes Ipmk a useful target for dissecting how inositol-derived messengers coordinate gene expression and cell fate decisions.
IPMK CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ipmk gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ipmk together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ipmk open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IPMK protein expression.
This CRISPR knockout system enables efficient generation of Ipmk-deficient cell models for investigation of IPMK signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ipmk exon(s) critical for IPMK function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ipmk genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IPMK CRISPR/Cas9 KO Plasmid (m) and IPMK CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ipmk locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IPMK HDR Plasmid (m) and IPMK HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ipmk homology arms to support homology-directed repair at defined Ipmk target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.