Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IP Receptor CRISPR/Cas9 KO Plasmid (h): sc-404135
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IP Receptor CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IP Receptor genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IP Receptor Antibody (B-6): sc-515139
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IP Receptor CRISPR/Cas9 KO Plasmid (h) | sc-404135 | 20 µg | $397.00 |
Overview
PTGIR encodes the human prostacyclin (IP) receptor, a G protein–coupled receptor that binds prostacyclin (PGI2) to regulate cAMP signaling and downstream PKA-dependent transcriptional programs. IP receptor activation modulates vascular tone, platelet responsiveness, and smooth muscle cell behavior, integrating eicosanoid biosynthesis with GPCR second-messenger pathways. PTGIR signaling also interfaces with inflammatory circuits and endothelial homeostasis through regulation of cytokine production and barrier function. Altered prostacyclin–IP receptor axis activity has been associated with cardiopulmonary and vascular dysfunction phenotypes, supporting its relevance in studies of thrombosis, pulmonary vascular biology, and inflammatory disease mechanisms.
IP Receptor CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PTGIR gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PTGIR together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PTGIR open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IP Receptor protein expression.
This CRISPR knockout system enables efficient generation of PTGIR-deficient cell models for investigation of IP Receptor signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PTGIR exon(s) critical for IP Receptor function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PTGIR genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IP Receptor CRISPR/Cas9 KO Plasmid (h) and IP Receptor CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PTGIR locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IP Receptor HDR Plasmid (h) and IP Receptor HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PTGIR homology arms to support homology-directed repair at defined PTGIR target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.