Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Integrin αX/ITGAX/CD11c Double Nickase Plasmid (h): sc-401765-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin αX/ITGAX/CD11c Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Integrin αX/ITGAX/CD11c Double Nickase Plasmid (h) and Integrin αX/ITGAX/CD11c Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ITGAX. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Integrin αX/ITGAX/CD11c Antibody (B-6): sc-46676
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin αX/ITGAX/CD11c Double Nickase Plasmid (h) | sc-401765-NIC | 20 µg | $410.00 | |||
Integrin αX/ITGAX/CD11c Double Nickase Plasmid (h2) | sc-401765-NIC-2 | 20 µg | $410.00 |
ITGAX encodes integrin αX (CD11c), which heterodimerizes with integrin β2 (CD18) to form complement receptor 4 (CR4), a key adhesion and recognition receptor on dendritic cells, monocytes, macrophages, and subsets of neutrophils. CD11c mediates leukocyte adhesion, migration, and phagocytosis by binding ligands such as iC3b-opsonized particles and fibrinogen, coordinating outside-in signaling that interfaces with cytoskeletal remodeling and inflammatory transcriptional programs. Through integrin-dependent signaling and crosstalk with innate immune receptors, ITGAX contributes to antigen uptake, cell–cell interactions, and shaping of adaptive immune responses. Altered CD11c expression and function are studied in contexts including chronic inflammation, autoimmunity, tumor-associated myeloid biology, and dysregulated tissue immune surveillance.
Integrin αX/ITGAX/CD11c Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ITGAX locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ITGAX. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ITGAX function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ITGAX-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.