Integrin αX/ITGAX/CD11c Antibody (B-6): sc-46676

Integrin αX Antibody (B-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects Integrin αX of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Integrin αX antibody (B-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Integrin αX, also known as CD11c, is a type 1 transmembrane protein that plays a crucial role in the immune system by forming a leukocyte-specific integrin when combined with the β2 chain, known as the inactivated-C3b receptor 4 (CR4). This integrin is essential for mediating the adherence of neutrophils and monocytes to activated endothelial cells, as well as facilitating the phagocytosis of complement-coated particles, which is vital for the clearance of pathogens and the initiation of immune responses. Abnormal expression of Integrin αX is notably associated with hairy cell leukemia, highlighting its importance in cancer biology and immune regulation. Transcription of Integrin αX is influenced by various proteins and DNA elements, including Sp1, AP-1 family members, and PU.1, which underscores the complexity of regulation in immune cells. Integrin αX is predominantly expressed on monocyte-derived dendritic cells, macrophages, and natural killer cells, and upon activation, dendritic cells located in tissues such as the skin, lungs, and gastrointestinal tract can migrate to lymphoid organs, where they interact with T and B cells to orchestrate adaptive immune responses.

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Integrin αX/ITGAX/CD11c Antibody (B-6) References:

1. Characteristics of fibrinogen binding to the domain of CD11c, an alpha subunit of p150,95.  |  Nham, SU. 1999. Biochem Biophys Res Commun. 264: 630-4. PMID: 10543983
2. Cutting edge: heat shock protein gp96 induces maturation and migration of CD11c+ cells in vivo.  |  Binder, RJ., et al. 2000. J Immunol. 165: 6029-35. PMID: 11086034
3. Human umbilical vein endothelial cells express complement receptor 1 (CD35) and complement receptor 4 (CD11c/CD18) in vitro.  |  Langeggen, H., et al. 2002. Inflammation. 26: 103-10. PMID: 12083416
4. CD11c gene expression in hairy cell leukemia is dependent upon activation of the proto-oncogenes ras and junD.  |  Nicolaou, F., et al. 2003. Blood. 101: 4033-41. PMID: 12576324
5. Relationships among murine CD11c(high) dendritic cell subsets as revealed by baseline gene expression patterns.  |  Edwards, AD., et al. 2003. J Immunol. 171: 47-60. PMID: 12816982
6. Estrogen preferentially promotes the differentiation of CD11c+ CD11b(intermediate) dendritic cells from bone marrow precursors.  |  Paharkova-Vatchkova, V., et al. 2004. J Immunol. 172: 1426-36. PMID: 14734718
7. CD11c+ dendritic cells are required for survival in murine polymicrobial sepsis.  |  Scumpia, PO., et al. 2005. J Immunol. 175: 3282-6. PMID: 16116220
8. TNF-alpha-dependent and -independent maturation of dendritic cells and recruited CD11c(int)CD11b+ Cells during oral Salmonella infection.  |  Sundquist, M. and Wick, MJ. 2005. J Immunol. 175: 3287-98. PMID: 16116221
9. Differentiation of CD11c+ CX3CR1+ cells in the small intestine requires Notch signaling.  |  Ishifune, C., et al. 2014. Proc Natl Acad Sci U S A. 111: 5986-91. PMID: 24711412
10. Circulating classical monocytes are associated with CD11c+ macrophages in human visceral adipose tissue.  |  Wouters, K., et al. 2017. Sci Rep. 7: 42665. PMID: 28198418
11. Deep Phenotyping of CD11c+ B Cells in Systemic Autoimmunity and Controls.  |  Rincon-Arevalo, H., et al. 2021. Front Immunol. 12: 635615. PMID: 33777025
12. Adenosine receptor 2a agonists target mouse CD11c+T-bet+ B cells in infection and autoimmunity.  |  Levack, RC., et al. 2022. Nat Commun. 13: 452. PMID: 35064115

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Integrin αX/ITGAX/CD11c Antibody (B-6) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-Integrin αX/ITGAX/CD11c Antibody (B-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Integrin αX/ITGAX/CD11c Antibody (B-6) Specifications

Alternate Product Name: Integrin αX

Product Clone Name: B-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 613-680

Epitope Target: Integrin αX

Epitope Species: human

Product Reactivity/Detection: Integrin αX

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for Integrin αX/ITGAX/CD11c Antibody (B-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin