Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Integrin αIIb/ITGA2B/CD41 Double Nickase Plasmid (h): sc-400614-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin αIIb/ITGA2B/CD41 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Integrin αIIb/ITGA2B/CD41 Double Nickase Plasmid (h) and Integrin αIIb/ITGA2B/CD41 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ITGA2B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Integrin αIIb/ITGA2B/CD41 Antibody (B-9): sc-365938
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin αIIb/ITGA2B/CD41 Double Nickase Plasmid (h) | sc-400614-NIC | 20 µg | $410.00 | |||
Integrin αIIb/ITGA2B/CD41 Double Nickase Plasmid (h2) | sc-400614-NIC-2 | 20 µg | $410.00 |
ITGA2B encodes integrin αIIb (CD41), a platelet- and megakaryocyte-enriched adhesion receptor that heterodimerizes with integrin β3 (αIIbβ3) to bind fibrinogen and other RGD-containing ligands. This integrin is a central mediator of platelet aggregation, cytoskeletal remodeling, and bidirectional outside-in/inside-out signaling that coordinates adhesion, spreading, and granule secretion. αIIbβ3 signaling interfaces with Src/FAK-dependent pathways, PI3K–AKT signaling, and actin regulatory networks to regulate hemostatic plug formation and thrombus stability. Genetic variation or altered expression of ITGA2B is associated with inherited platelet function disorders and is widely studied in the context of dysregulated thrombosis and bleeding phenotypes.
Integrin αIIb/ITGA2B/CD41 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ITGA2B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ITGA2B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ITGA2B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ITGA2B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.