Integrin beta 1 Antibody (P5D2) recommended for detection of Integrin β1 of human origin by WB, IP, IF and FCM

Integrin β1 Antibody (P5D2): sc-13590

Integrin β1 Antibody (P5D2) is rated 4.7 out of 5 by 10.
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Datasheets

    • Integrin β1 Antibody (P5D2) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against human epidermal keratinocytes
    • Integrin beta 1 Antibody (P5D2) is recommended for detection of Integrin β1 of human origin by WB, IP, IF and FCM
    • Anti-Integrin beta 1 Antibody (P5D2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also provided azide-free for blocking, sc-13590 L, 200 µg/0.1 ml
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Integrin β1 (P5D2): sc-13590.

What is the recommended secondary reagent for Western blot?

Asked by: Trav11
Thank you for your question. We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-8301 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Service 11
Date published: 2017-01-16

Is Integrin β1 (P5D2): sc-13590 able to detect Integrin β1 under reducing conditions?

Asked by: Trav11
Thank you for your question. The Integrin β1 Antibody (P5D2): sc-13590 is only capable of Integrin β1 detection under non-reducing condition. If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 15
Date published: 2017-01-13

Hi. What does the 'L' mean in the catalog number of 'sc-13590L'? Can I consider this 'sc-13590L' product as same antibody as 'sc-13590' but just without azide? It seems that the concentration of 'sc-13590L' is 10 times higher than 'sc-13590'. Am I right?

Asked by: Jihyun
Thank you for your inquiry. The L form means that this product is provided without gelatin or sodium azide in the buffer, just in PBS. This product is 10 times more concentrated than the standard product.
Answered by: Technical Service
Date published: 2016-12-01
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Rated 5 out of 5 by from Worked great for blocking Used for blocking ITGB1 at 2.5ug/ml over several days. Worked great, probably ok down to 1ug/ml for short term.
Date published: 2018-02-02
Rated 5 out of 5 by from Crispy Signals! Worked excellent for my flow cytometry application (BD FACS Canto).
Date published: 2017-09-13
Rated 5 out of 5 by from Works great We had been used b1 antibodies from another source before but noticed the results are not consistent, varying with batches of antibodies. This antibody works great on human kidney tissues. The IHC results look identical as another b1 antibody (K-20): sc-18887.
Date published: 2017-06-06
Rated 5 out of 5 by from Great Integrin beta-1 antibody PE and FITC direct conjugates great for flow. Strong data in human samples and well published for WB, IF, and flow cytometry.
Date published: 2017-01-23
Rated 5 out of 5 by from Very nice product Strong FCM, IF and WB results; also 4B7R alternative monoclonal from scbt
Date published: 2017-01-15
Rated 4 out of 5 by from Used for WB and blocking integrin Used for WB and blocking integrin - worked well.
Date published: 2015-10-16
Rated 5 out of 5 by from Used for immunofluorescence of HSCs fixed Used for immunofluorescence of HSCs fixed in 4% pfa for 15 mins.
Date published: 2015-07-31
Rated 4 out of 5 by from Western blot analysis of U Western blot analysis of U-937 gave strong signal. -SCBT QC
Date published: 2015-04-07
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