Integrin α6/ITGA6/CD49f Antibody (GOH3): sc-19622

Integrin α6 Antibody (GOH3) is a rat monoclonal IgG2a antibody raised against an extracellular epitope of human Integrin α6, detecting this protein in mouse, rat, and human samples by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Integrin α6, also known as CD49f or ITGA6, is a transmembrane protein that pairs with β subunits to form functional integrin receptors crucial for cell adhesion to the extracellular matrix, particularly by binding to laminin. This interaction plays a vital role in biological processes such as cell growth, differentiation, migration, and apoptosis, which are essential for tissue development and wound healing. Dysregulation of Integrin α6 expression or function has been linked to tumor progression and metastasis, making Integrin α6 a significant target in cancer research. Anti-Integrin α6 antibody (GOH3) is available in non-conjugated form and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, offering versatility for different experimental applications. Integrins, comprising 16 α and 8 β subunits that heterodimerize into over 20 different receptors, are integral to cell-cell and cell-matrix interactions. By binding to extracellular matrix components like fibronectin, collagen, and vitronectin, or soluble ligands like fibrinogen, integrins facilitate cell aggregation and signal transduction. Activation of integrin-mediated responses requires both receptor clustering and ligand occupancy, leading to cytoskeletal organization and enhanced cell adhesion. Given its pivotal role in cell signaling and adhesion, Integrin α6 serves as a valuable marker in biomedical research, particularly in studies involving stem cells, cancer, and tissue regeneration.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Integrin α6/ITGA6/CD49f Antibody (GOH3) References:

1. Integrin (alpha 6 beta 4) regulation of eIF-4E activity and VEGF translation: a survival mechanism for carcinoma cells.  |  Chung, J., et al. 2002. J Cell Biol. 158: 165-74. PMID: 12105188
2. An extracellular site on tetraspanin CD151 determines alpha 3 and alpha 6 integrin-dependent cellular morphology.  |  Kazarov, AR., et al. 2002. J Cell Biol. 158: 1299-309. PMID: 12356873
3. Alpha 6 beta 4 integrin regulates keratinocyte chemotaxis through differential GTPase activation and antagonism of alpha 3 beta 1 integrin.  |  Russell, AJ., et al. 2003. J Cell Sci. 116: 3543-56. PMID: 12865436
4. The Met receptor and alpha 6 beta 4 integrin can function independently to promote carcinoma invasion.  |  Chung, J., et al. 2004. J Biol Chem. 279: 32287-93. PMID: 15161909
5. Integrins: versatility, modulation, and signaling in cell adhesion.  |  Hynes, RO. 1992. Cell. 69: 11-25. PMID: 1555235
6. Integrin α6 (CD49f), The Microenvironment and Cancer Stem Cells.  |  Bigoni-Ordóñez, GD., et al. 2019. Curr Stem Cell Res Ther. 14: 428-436. PMID: 30280675
7. Integrin α6-Targeted Positron Emission Tomography Imaging of Colorectal Cancer.  |  Xiao, YT., et al. 2019. ACS Omega. 4: 15560-15566. PMID: 31572857
8. The calcium channel TRPC6 promotes chemotherapy-induced persistence by regulating integrin α6 mRNA splicing.  |  Mukhopadhyay, D., et al. 2023. Cell Rep. 42: 113347. PMID: 37910503
9. Integrins and signal transduction pathways: the road taken.  |  Clark, EA. and Brugge, JS. 1995. Science. 268: 233-9. PMID: 7716514
10. Synergistic roles for receptor occupancy and aggregation in integrin transmembrane function.  |  Miyamoto, S., et al. 1995. Science. 267: 883-5. PMID: 7846531
11. Epithelial integrins.  |  Sheppard, D. 1996. Bioessays. 18: 655-60. PMID: 8760339
12. Cooperation between soluble factors and integrin-mediated cell anchorage in the control of cell growth and differentiation.  |  Juliano, R. 1996. Bioessays. 18: 911-7. PMID: 8939069

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Integrin α6/ITGA6/CD49f Antibody (GOH3) has 60 citations in a variety of scientific publications.

Primary antibodies like Anti-Integrin α6/ITGA6/CD49f Antibody (GOH3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Integrin α6/ITGA6/CD49f Antibody (GOH3) Specifications

Alternate Product Name: Integrin α6

Product Clone Name: GOH3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids:

Epitope Target: Integrin α6

Epitope Species: human

Product Reactivity/Detection: Integrin α6

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 60 citations in various scientific publications

Available Conjugates/Forms for Integrin α6/ITGA6/CD49f Antibody (GOH3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, L: Azide-Free, PE: Phycoerythrin