Integrin α4/ITGA4/CD49d Antibody (B-2): sc-376334

Integrin α4 Antibody (B-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects Integrin alpha 4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Integrin alpha 4 antibody (B-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Integrin alpha 4 plays a crucial role in cell adhesion and migration by forming heterodimers with beta subunits, which allows interaction with extracellular matrix components and other cell surface receptors. This interaction is vital for processes such as leukocyte trafficking and tissue infiltration, making Integrin alpha 4 a key player in immune responses and inflammation. The ability of Integrin alpha 4 to bind to ligands like fibronectin and vascular cell adhesion molecule-1 (VCAM-1) facilitates the adhesion of immune cells to the endothelium, which is essential for the recruitment of these cells to sites of injury or infection. Using anti-Integrin alpha 4 antibody (B-2) in research can provide insights into the mechanisms of cell adhesion and migration, which are fundamental to understanding various physiological and pathological processes.

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Integrin α4/ITGA4/CD49d Antibody (B-2) References:

1. Antibodies to CD44 and integrin alpha4, but not L-selectin, prevent central nervous system inflammation and experimental encephalomyelitis by blocking secondary leukocyte recruitment.  |  Brocke, S., et al. 1999. Proc Natl Acad Sci U S A. 96: 6896-901. PMID: 10359810
2. Effect of antibody against integrin alpha4 on bleomycin-induced pulmonary fibrosis in mice.  |  Wang, Q., et al. 2000. Biochem Pharmacol. 60: 1949-58. PMID: 11108812
3. Preferential production of interferon-gamma by CD4+ T cells expressing the homing receptor integrin alpha4/beta7.  |  Abramson, O., et al. 2001. Immunology. 103: 155-63. PMID: 11412302
4. Functional differences between integrin alpha4 and integrins alpha5/alphav in modulating the motility of human oral squamous carcinoma cells in response to the V region and heparin-binding domain of fibronectin.  |  Zhang, Y., et al. 2004. Exp Cell Res. 295: 48-58. PMID: 15051489
5. Identification of integrin-alpha4, Rb1, and syncytin a as murine placental target genes of the transcription factor GCMa/Gcm1.  |  Schubert, SW., et al. 2008. J Biol Chem. 283: 5460-5. PMID: 18167345
6. CpG island hypermethylation of E-cadherin (CDH1) and integrin alpha4 is associated with recurrence of early stage esophageal squamous cell carcinoma.  |  Lee, EJ., et al. 2008. Int J Cancer. 123: 2073-9. PMID: 18697202
7. Guanylate binding protein-1 inhibits spreading and migration of endothelial cells through induction of integrin alpha4 expression.  |  Weinländer, K., et al. 2008. FASEB J. 22: 4168-78. PMID: 18697840
8. The PI3K/Akt pathway upregulates Id1 and integrin α4 to enhance recruitment of human ovarian cancer endothelial progenitor cells.  |  Su, Y., et al. 2010. BMC Cancer. 10: 459. PMID: 20796276
9. Sjögren's syndrome associated dry eye in a mouse model is ameliorated by topical application of integrin α4 antagonist GW559090.  |  Contreras-Ruiz, L., et al. 2016. Exp Eye Res. 143: 1-8. PMID: 26463157
10. Simulated physiological stretch increases expression of extracellular matrix proteins in human bladder smooth muscle cells via integrin α4/αv-FAK-ERK1/2 signaling pathway.  |  Chen, S., et al. 2017. World J Urol. 35: 1247-1254. PMID: 28013345

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Integrin α4/ITGA4/CD49d Antibody (B-2) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Integrin α4/ITGA4/CD49d Antibody (B-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Integrin α4/ITGA4/CD49d Antibody (B-2) Specifications

Alternate Product Name: Integrin alpha-4

Product Clone Name: B-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1013-1032

Epitope Target: Integrin α4

Epitope Species: human

Product Reactivity/Detection: Integrin α4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Integrin α4/ITGA4/CD49d Antibody (B-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Integrin α4/ITGA4/CD49d antibody is Integrin α4/ITGA4/CD49d Antibody (A-7): sc-365209.