Integrin β7/ITGB7 Double Nickase Plasmid (h): sc-404928-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin β7/ITGB7 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Integrin β7/ITGB7 Double Nickase Plasmid (h) and Integrin β7/ITGB7 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ITGB7. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Integrin β7/ITGB7 Antibody (D-5): sc-515397
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin β7/ITGB7 Double Nickase Plasmid (h) | sc-404928-NIC | 20 µg | $410.00 | |||
Integrin β7/ITGB7 Double Nickase Plasmid (h2) | sc-404928-NIC-2 | 20 µg | $410.00 |
ITGB7 encodes integrin β7, a transmembrane adhesion receptor subunit that pairs with α4 or αE to form α4β7 and αEβ7, coordinating leukocyte trafficking and retention in tissue microenvironments. These integrins engage ligands such as MAdCAM-1 and E-cadherin to support cell adhesion, migration, and immune cell positioning, coupling extracellular binding to cytoskeletal remodeling and bidirectional “inside-out/outside-in” signaling. ITGB7-dependent pathways contribute to lymphocyte homing to gut-associated lymphoid tissues and regulation of mucosal immune responses. Dysregulated integrin signaling and altered ITGB7 expression are studied in inflammatory disorders and immune-oncology contexts where cell localization, adhesion dynamics, and immune surveillance are perturbed.
Integrin β7/ITGB7 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ITGB7 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ITGB7. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ITGB7 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ITGB7-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.