Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Integrin α6 Antibody (541A11): sc-47772. Western blot analysis of Integrin α6 expression in HeLa (A), RAW 264.7 (B), F9 (C) and RBL-1 (D) whole cell lysates.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Integrin α6/ITGA6/CD49f Double Nickase Plasmid (m): sc-421164-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin α6/ITGA6/CD49f Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Integrin α6/ITGA6/CD49f Double Nickase Plasmid (m) and Integrin α6/ITGA6/CD49f Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Itga6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Integrin α6/ITGA6/CD49f Antibody (F-6): sc-374057
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin α6/ITGA6/CD49f Double Nickase Plasmid (m) | sc-421164-NIC | 20 µg | $410.00 |