INSR/Insulin Receptor β Antibody (CT-3): sc-57342

Insulin Rβ Antibody (CT-3) is a mouse monoclonal IgG1 kappa kappa light chain antibody, specifically designed to target the C-terminus of the human insulin receptor beta subunit. Insulin Rβ monoclonal antibody (CT-3) reliably detects the insulin receptor beta protein from human, mouse, and rat origins across multiple applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP), while demonstrating no cross-reactivity with insulin-like growth factor (IGF) receptors, ensuring precise and specific binding. The insulin receptor (IR) itself is a vital heterodimeric protein complex composed of extracellular alpha subunits and transmembrane beta subunits connected via disulfide bonds. Upon insulin binding, IR undergoes autophosphorylation of its intracellular tyrosine kinase domains, activating a cascade of signaling proteins such as IRS-1, IRS-2, PI3K, and GRB2. These interactions initiate key molecular pathways that regulate glucose uptake, glycogen synthesis, and various aspects of cell growth and metabolism. By targeting the beta subunit, anti-Insulin Rβ antibody (CT-3) facilitates the investigation of these intricate signaling mechanisms and protein interactions, providing valuable insights into insulin signaling pathways. Understanding the role of IR is crucial in studying metabolic disorders such as Type 1 diabetes, an autoimmune condition leading to the destruction of insulin-producing cells, and Type 2 diabetes, characterized by cellular resistance to insulin action. Insulin Rβ (CT-3) monoclonal antibody serves as an essential tool for researchers exploring the molecular pathways and protein interactions underpinning insulin signaling and its implications in various diseases. Available in both unconjugated form and multiple conjugated variants, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, Insulin Rβ (CT-3) antibody offers versatility for diverse research applications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

INSR/Insulin Receptor β Antibody (CT-3) References:

1. Signalling through the insulin receptor.  |  Whitehead, JP., et al. 2000. Curr Opin Cell Biol. 12: 222-8. PMID: 10712920
2. The insulin receptor: from protein sequence to structure.  |  Marino-Buslje, C., et al. 1999. Biochem Soc Trans. 27: 715-26. PMID: 10917671
3. Mechanism of transmembrane signaling: insulin binding and the insulin receptor.  |  Ottensmeyer, FP., et al. 2000. Biochemistry. 39: 12103-12. PMID: 11015187
4. Insulin receptor--structural and functional characteristics.  |  Perz, M. and Torlińska, T. 2001. Med Sci Monit. 7: 169-77. PMID: 11208515
5. Insulin receptor variant forms and type 2 diabetes mellitus.  |  Sesti, G. 2000. Pharmacogenomics. 1: 49-61. PMID: 11258596
6. Extended longevity in mice lacking the insulin receptor in adipose tissue.  |  Blüher, M., et al. 2003. Science. 299: 572-4. PMID: 12543978
7. Insulin signaling in the nervous system regulates ethanol intoxication in Drosophila melanogaster.  |  Corl, AB., et al. 2005. Nat Neurosci. 8: 18-9. PMID: 15592467
8. Lack of the architectural factor HMGA1 causes insulin resistance and diabetes in humans and mice.  |  Foti, D., et al. 2005. Nat Med. 11: 765-73. PMID: 15924147
9. The first three domains of the insulin receptor differ structurally from the insulin-like growth factor 1 receptor in the regions governing ligand specificity.  |  Lou, M., et al. 2006. Proc Natl Acad Sci U S A. 103: 12429-34. PMID: 16894147

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-INSR/Insulin Receptor β Antibody (CT-3) has 64 citations in a variety of scientific publications.

Primary antibodies like Anti-INSR/Insulin Receptor β Antibody (CT-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

INSR/Insulin Receptor β Antibody (CT-3) Specifications

Alternate Product Name: Insulin Receptor Beta

Product Clone Name: CT-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: insulin receptor

Epitope Species: human

Product Reactivity/Detection: insulin Rβ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 64 citations in various scientific publications

Available Conjugates/Forms for INSR/Insulin Receptor β Antibody (CT-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin