insulin R alpha Antibody (83-12) recommended for detection of insulin Rα of human origin by WB and IP

insulin Rα Antibody (83-12): sc-57343

insulin Rα Antibody (83-12) is rated 2.5 out of 5 by 2.
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Datasheets

    • Anti-insulin Rα Antibody (83-12) is a mouse monoclonal IgG1 (kappa light chain) insulin Rα antibody provided at 200 µg/ml
    • raised against insulin Rα of human origin
    • insulin R alpha Antibody (83-12) is recommended for detection of insulin Rα of human origin by WB and IP
    • Anti-insulin R alpha Antibody (83-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of insulin Rα (83-12): sc-57343.
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Can sc-57343: insulin Rα(83-12) differs insulin receptor A from insulin receptor B in western blot? It seems that there is few specific antibody of IR-A.

Asked by: YouMaru
Thank you for your question. This monoclonal insulin Rα Antibody (83-12): sc-57343 is specific for insulin Rα and recommended for use in immunoprecipitation (IP).
Answered by: Technical Support
Date published: 2018-12-06

What is the recommended secondary reagent to be used with sc-57343: insulin Rα (83-12) mouse monoclonal primary?

Asked by: TinTin
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-04-12
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Rated 1 out of 5 by from IHC I tried both antibodies on mouse hypothalamic tissue. None of them work for IHC
Date published: 2017-09-17
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