INSR/Insulin Receptor α Antibody (83-12): sc-57343

Insulin Rα Antibody (83-12) is a mouse monoclonal IgG1 kappa light chain antibody engineered to target the alpha subunit of the human insulin receptor (IR) with exceptional precision, making anti-insulin Rα antibody (83-12) an essential tool for applications such as western blotting (WB) and immunoprecipitation (IP). Insulin Rα monoclonal antibody (83-12) is raised against the insulin Rα of human origin, ensuring reliable detection of the insulin receptor in various experimental settings, and insulin Rα (83-12) antibody also exhibits reactivity with insulin receptors from additional species, including bovine and porcine, enhancing its versatility for comparative studies. The insulin receptor is a crucial heterotetrameric protein composed of two extracellular alpha subunits and two transmembrane beta subunits, which are connected by disulfide bonds. The alpha subunits are responsible for binding insulin, initiating conformational changes that activate the receptor′s intrinsic tyrosine kinase activity in the beta subunits. This structural configuration is vital for the receptor′s function in mediating insulin′s metabolic effects, such as facilitating glucose uptake into cells, promoting glycogen synthesis, and regulating various aspects of cellular metabolism and growth. Upon insulin binding, the receptor undergoes autophosphorylation of its tyrosine kinase domains, triggering a cascade of downstream signaling pathways that enhance glucose transport, increase cell permeability to monosaccharides, amino acids, and fatty acids, and maintain metabolic homeostasis. The precise structure of the insulin receptor is essential for its high-affinity insulin binding and effective signal transduction, making insulin receptor a key player in maintaining normal blood glucose levels and overall metabolic balance. Dysregulation or structural abnormalities of the insulin receptor can lead to insulin resistance and are implicated in metabolic disorders such as type 2 diabetes mellitus, highlighting the importance of studying the insulin receptor′s structure and function.

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INSR/Insulin Receptor α Antibody (83-12) References:

1. Signalling through the insulin receptor.  |  Whitehead, JP., et al. 2000. Curr Opin Cell Biol. 12: 222-8. PMID: 10712920
2. The insulin receptor: from protein sequence to structure.  |  Marino-Buslje, C., et al. 1999. Biochem Soc Trans. 27: 715-26. PMID: 10917671
3. Mechanism of transmembrane signaling: insulin binding and the insulin receptor.  |  Ottensmeyer, FP., et al. 2000. Biochemistry. 39: 12103-12. PMID: 11015187
4. Insulin receptor--structural and functional characteristics.  |  Perz, M. and Torlińska, T. 2001. Med Sci Monit. 7: 169-77. PMID: 11208515
5. Insulin receptor variant forms and type 2 diabetes mellitus.  |  Sesti, G. 2000. Pharmacogenomics. 1: 49-61. PMID: 11258596
6. Targeted inactivation of the IL-4 receptor alpha chain I4R motif promotes allergic airway inflammation.  |  Blaeser, F., et al. 2003. J Exp Med. 198: 1189-200. PMID: 14557412
7. Estrogen regulates insulin-like growth factor 1, platelet-derived growth factor A and B, and their receptors in the vascular wall.  |  Savolainen-Peltonen, H., et al. 2004. Transplantation. 77: 35-42. PMID: 14724432
8. [A study of fluoride and dexamethasone affecting the expression of growth factors in osteoblast].  |  Ge, LH., et al. 2001. Shanghai Kou Qiang Yi Xue. 10: 336-8, 345. PMID: 14993967
9. Distinct expression pattern of insulin-like growth factor family in rodent taste buds  |  Yuko Suzuki, et al. 2005. JCN. 482: 74-84.
10. Ultrastructural immunolocalization of IGF-1 and insulin receptors in rat pituitary culture: evidence of a functional interaction between gonadotroph and lactotroph cells  |  Silvina Gutierrez, et al. 2006. Springer Link. 327: 121-132.
11. Proteinase Activity and Receptor Cleavage: Mechanism for Insulin Resistance in the Spontaneously Hypertensive Rat  |  Frank A. DeLano, et al. 2008. AHA/ASA. 52.
12. IGF-I and INS receptor expression in the salivary glands of diabetic Nod mice submitted to long-term insulin treatment  |  Eduardo Jose Caldeira and Valeria Helena Cagnon. 2008. ELSEVIER. 32: 16-21.

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-INSR/Insulin Receptor α Antibody (83-12) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-INSR/Insulin Receptor α Antibody (83-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

INSR/Insulin Receptor α Antibody (83-12) Specifications

Alternate Product Name: Insulin Receptor Alpha

Product Clone Name: 83-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: insulin Rα

Epitope Species: human

Product Reactivity/Detection: insulin Rα

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for INSR/Insulin Receptor α Antibody (83-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred INSR/Insulin Receptor β antibody is INSR/Insulin Receptor β Antibody (CT-3): sc-57342.