INSC Antibody (G-6): sc-514398

INSC Antibody (G-6) is a mouse monoclonal IgM antibody that detects INSC protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-INSC antibody (G-6) is available as the non-conjugated form. INSC protein, also known as inscuteable homolog, is a 579 amino acid protein that plays a crucial role in cellular processes by interacting with key proteins such as PAR-3, AGS3, nucleobindin, and PARD3B. This interaction is significant as INSC may function as an adapter that links the Par3 complex to the AGS3/nucleobindin complex, which is essential for proper spindle orientation during mitosis. INSC is involved in regulating differentiation and cell proliferation in the developing nervous system, influencing asymmetric division of fibroblasts, and participating in stratification of squamous epithelium. Localized primarily in the cytoplasm, INSC is expressed in fetal cochlea and exists as five isoforms due to alternative splicing events. Isoform 1 is widely expressed across various tissues, with notably higher levels in liver, kidney, and small intestine, while Isoform 2 shows abundant expression in small intestine, with lower levels detected in lung and pancreas.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

INSC Antibody (G-6) References:

1. PAR3beta, a novel homologue of the cell polarity protein PAR3, localizes to tight junctions.  |  Kohjima, M., et al. 2002. Biochem Biophys Res Commun. 299: 641-6. PMID: 12459187
2. Identification and characterization of human Inscuteable gene in silico.  |  Katoh, M. and Katoh, M. 2003. Int J Mol Med. 11: 111-6. PMID: 12469229
3. Mammalian inscuteable regulates spindle orientation and cell fate in the developing retina.  |  Zigman, M., et al. 2005. Neuron. 48: 539-45. PMID: 16301171
4. Two forms of human Inscuteable-related protein that links Par3 to the Pins homologues LGN and AGS3.  |  Izaki, T., et al. 2006. Biochem Biophys Res Commun. 341: 1001-6. PMID: 16458856
5. Distribution of activator of G-protein signaling 3 within the aggresomal pathway: role of specific residues in the tetratricopeptide repeat domain and differential regulation by the AGS3 binding partners Gi(alpha) and mammalian inscuteable.  |  Vural, A., et al. 2010. Mol Cell Biol. 30: 1528-40. PMID: 20065032
6. Asymmetrical cell division and differentiation are not dependent upon stratification in a corneal epithelial cell line.  |  Gómez-Flores, E., et al. 2011. J Cell Physiol. 226: 700-9. PMID: 20717959

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-INSC Antibody (G-6) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-INSC Antibody (G-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

INSC Antibody (G-6) Specifications

Alternate Product Name: INSC

Product Clone Name: G-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 251-274

Epitope Target: INSC

Epitope Species: human

Product Reactivity/Detection: INSC

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for INSC Antibody (G-6): P: Blocking Peptide