ING2 Double Nickase Plasmid (h): sc-404193-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ING2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ING2 Double Nickase Plasmid (h) and ING2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ING2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ING2 Antibody (B-5): sc-271544
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ING2 Double Nickase Plasmid (h) | sc-404193-NIC | 20 µg | $410.00 | |||
ING2 Double Nickase Plasmid (h2) | sc-404193-NIC-2 | 20 µg | $410.00 |
ING2 (inhibitor of growth family member 2) encodes a chromatin-associated tumor suppressor protein that functions as an epigenetic reader through its PHD finger, recognizing H3K4me3 to couple histone marks with transcriptional outcomes. ING2 participates in DNA damage responses and cell-cycle control by modulating p53-dependent programs and cooperating with histone acetylation/deacetylation complexes that influence chromatin accessibility. Through these activities, ING2 impacts pathways governing apoptosis, senescence, and genome stability. Altered ING2 expression or function has been associated with dysregulated chromatin remodeling and has been reported across multiple cancer-related contexts, making it a useful node for mechanistic studies of epigenetic control.
ING2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ING2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ING2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ING2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ING2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.